Anti-WSX-1 antibody (ab5997)
Key features and details
- Rabbit polyclonal to WSX-1
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-WSX-1 antibody
See all WSX-1 primary antibodies -
Description
Rabbit polyclonal to WSX-1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human WSX-1 aa 44-59 (N terminal).
Sequence:GSAGPLQCYGVGPLGD
-
Positive control
- Human spleen tissue lysate.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab5997 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 70 kDa.
|
|
ICC/IF |
Use a concentration of 20 µg/ml.
|
|
IHC-P |
Use a concentration of 10 µg/ml.
|
Notes |
---|
WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 70 kDa. |
ICC/IF
Use a concentration of 20 µg/ml. |
IHC-P
Use a concentration of 10 µg/ml. |
Target
-
Function
Receptor for IL27. Requires IL6ST/gp130 to mediate signal transduction in response to IL27. This signaling system acts through STAT3 and STAT1. Involved in the regulation of Th1-type immune responses. Also appears to be involved in innate defense mechanisms. -
Tissue specificity
Highly expressed in lymphoid tissues such as spleen, lymph nodes and peripheral blood leukocytes. Weakly expressed in other tissues examined including heart, brain, fetal and adult lung, liver, skeletal muscle, kidney, pancreas, prostate, testis, ovary, small intestine, kidney and colon. In the lymphoid system, higher level expression in CD4+ T-cell subsets than in CD8+ T-cell subsets. Also weaker expression in CD19+ B-cells and monocytes. -
Sequence similarities
Belongs to the type I cytokine receptor family. Type 2 subfamily.
Contains 3 fibronectin type-III domains. -
Domain
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 9466 Human
- Omim: 605350 Human
- SwissProt: Q6UWB1 Human
- Unigene: 132781 Human
-
Alternative names
- Class I cytokine receptor antibody
- CRL1 antibody
- Cytokine receptor-like 1 antibody
see all
Images
-
Western blot analysis of WSX-1 expression in human spleen tissue lysates with ab5997 at 1 µg /ml. Western blot analysis of WSX-1 expression in human spleen tissue lysates with ab5997 at 1 µg /ml.
-
ab5997 at 10µg/ml staining WSX-1 in human spleen tissue by IHC
-
Immunofluorescence of WSX-1 in A549 cells using ab5997 at 20 ug/ml.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab5997 has been referenced in 2 publications.
- Seita J et al. Interleukin-27 directly induces differentiation in hematopoietic stem cells. Blood 111:1903-12 (2008). PubMed: 18042804
- Yoshimura T et al. Two-sided roles of IL-27: induction of Th1 differentiation on naive CD4+ T cells versus suppression of proinflammatory cytokine production including IL-23-induced IL-17 on activated CD4+ T cells partially through STAT3-dependent mechanism. J Immunol 177:5377-85 (2006). PubMed: 17015723