Overview

  • Product name

    Anti-TCEA1 antibody [EPR14820(B)]
    See all TCEA1 primary antibodies
  • Description

    Rabbit monoclonal [EPR14820(B)] to TCEA1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human TCEA1 aa 100-200. The exact sequence is proprietary.
    Database link: P23193

  • Positive control

    • K562, HeLa, and SP2/0 cell lysate. Human lung adenocarcinoma and colon tissue. HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab184181 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
ICC/IF 1/250.
IP 1/40 - 1/60.
IHC-P 1/50 - 1/100.

Target

  • Function

    Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.
  • Involvement in disease

    Note=A chromosomal aberration involving TCEA1 may be a cause of salivary gland pleiomorphic adenomas (PA) [181030]. Pleiomorphic adenomas are the most common benign epithelial tumors of the salivary gland. Translocation t(3;8)(p21;q12) with PLAG1.
  • Sequence similarities

    Belongs to the TFS-II family.
    Contains 1 TFIIS central domain.
    Contains 1 TFIIS N-terminal domain.
    Contains 1 TFIIS-type zinc finger.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • GTF2S antibody
    • SII antibody
    • TCEA antibody
    • Tcea1 antibody
    • TCEA1_HUMAN antibody
    • TF2S antibody
    • TFIIS antibody
    • Transcription elongation factor A (SII) 1 antibody
    • Transcription elongation factor A protein 1 antibody
    • Transcription elongation factor A, 1 antibody
    • Transcription elongation factor S-II protein 1 antibody
    • Transcription elongation factor TFIIS.o antibody
    see all

Images

  • All lanes : Anti-TCEA1 antibody [EPR14820(B)] (ab184181) at 1/10000 dilution

    Lane 1 : K652 lysate
    Lane 2 : HeLa lysate
    Lane 3 : SP2/0 lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 34 kDa

  • Immunoprecipitation analysis of HeLa cell lysate labeling TCEA1 with ab184181 at 1/50 dilution.  Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated used as secondary antibody at 1/1000 dilution.

  • Immunohistochemistry analysis of Human lung adenocarcinoma tissue labeling TCEA1 with ab184181 at 1/100 dilution. Secondary antibody HRP conjugated Rabbit IgG. Counterstained with Hematoxylin.

  • Immunohistochemistry analysis of Human colon tissue labeling TCEA1 with ab184181 at 1/100 dilution. Secondary antibody HRP conjugated Rabbit IgG. Counterstained with Hematoxylin.

  • Immunocytochemistry analysis of a HeLa cells labeling TCEA1 with ab184181 at 1/250 dilution. Secondary antibody Goat anti rabbit IgG (Alexa Fluor®488) at a 1/200 dilution. Dapi counterstain shown in blue.

References

ab184181 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab184181.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up