Product nameAnti-TCEA1 antibody [EPR14821]
See all TCEA1 primary antibodies
DescriptionRabbit monoclonal [EPR14821] to TCEA1
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, Flow Cyt, IPmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human TCEA1 aa 200-300. The exact sequence is proprietary.
Database link: Q29RL9
- K562, HeLa, SP2/0 and HUVEC cell lysate; Human bladder transitional cell carcinoma tissue; HeLa, HUVEC cells
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab185947 in the following tested applications.
|WB||1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).|
|IHC-P||1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IP||1/30 - 1/50.|
FunctionNecessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.
Involvement in diseaseNote=A chromosomal aberration involving TCEA1 may be a cause of salivary gland pleiomorphic adenomas (PA) . Pleiomorphic adenomas are the most common benign epithelial tumors of the salivary gland. Translocation t(3;8)(p21;q12) with PLAG1.
Sequence similaritiesBelongs to the TFS-II family.
Contains 1 TFIIS central domain.
Contains 1 TFIIS N-terminal domain.
Contains 1 TFIIS-type zinc finger.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- GTF2S antibody
- SII antibody
- TCEA antibody
All lanes : Anti-TCEA1 antibody [EPR14821] (ab185947) at 1/10000 dilution
Lane 1 : K562 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : SP2/0 cell lysate
Lane 4 : HUVEC cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 34 kDa
Immunohistochemical analysis of paraffin embedded Human bladder transitional cell carcinoma tissue labeling TCEA1 with ab185947 at 1/500 dilution. Counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunofluorescent staining of -20 °C acetone fixed HeLa cells labeling TCEA1 with ab185947 at 1/500 dilution (red; left image) and DAPI nuclear staining (blue; right image). Secondary staining with Goat anti Rabbit IgG (Alexa Fluor® 555) at a 1/200 dilution.
Immunofluorescent staining of 4% paraformaldehyde fixed HUVEC cells labeling TCEA1 with ab185947 at 1/500 dilution (red; left image) and DAPI nuclear staining (blue; right image). Secondary staining with Goat anti Rabbit IgG (Alexa Fluor® 555) at a 1/200 dilution.
Flow cytometric analysis of 2% paraformaldehyde HeLa cells labeling TCEA1 with ab185947 at 1/250 dilution (red) compared to a Rabbit monoclonal IgG isotype control antibody (green). Secondary staining with Goat anti Rabbit IgG (FITC) at a 1/150 dilution.
Immunoprecipitation analysis of HeLa cell lysate labeling TCEA1 with ab185947 at 1/50 dilution. Secondary staining with Goat anti Rabbit IgG (H+L) Peroxidase conjugated antibody at a 1/1000 dilution.
ab185947 has not yet been referenced specifically in any publications.