Recombinant
RabMAb

Recombinant Anti-TCEA1 antibody [EPR15036-16] - N-terminal (ab184159)

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Overview

  • Product name

    Anti-TCEA1 antibody [EPR15036-16] - N-terminal
    See all TCEA1 primary antibodies

  • Description

    Rabbit monoclonal [EPR15036-16] to TCEA1 - N-terminal

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human TCEA1 aa 1-150 (N terminal). The exact sequence is proprietary.
    Database link: P23193

  • Positive control

    • Jurkat, K562, 293 and HUVEC lysates. HUVEC cells.

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab184159 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 34, 38 kDa (predicted molecular weight: 34 kDa).
IP 1/60 - 1/80.
ICC/IF 1/250.

Target

  • Function

    Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.

  • Involvement in disease

    Note=A chromosomal aberration involving TCEA1 may be a cause of salivary gland pleiomorphic adenomas (PA) [181030]. Pleiomorphic adenomas are the most common benign epithelial tumors of the salivary gland. Translocation t(3;8)(p21;q12) with PLAG1.

  • Sequence similarities

    Belongs to the TFS-II family.
    Contains 1 TFIIS central domain.
    Contains 1 TFIIS N-terminal domain.
    Contains 1 TFIIS-type zinc finger.

  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • GTF2S antibody
    • SII antibody
    • TCEA antibody
    • Tcea1 antibody
    • TCEA1_HUMAN antibody
    • TF2S antibody
    • TFIIS antibody
    • Transcription elongation factor A (SII) 1 antibody
    • Transcription elongation factor A protein 1 antibody
    • Transcription elongation factor A, 1 antibody
    • Transcription elongation factor S-II protein 1 antibody
    • Transcription elongation factor TFIIS.o antibody
    see all

Images

  • Western blot - Anti-TCEA1 antibody [EPR15036-16] - N-terminal (ab184159)
    Western blot - Anti-TCEA1 antibody [EPR15036-16] - N-terminal (ab184159)
    All lanes : Anti-TCEA1 antibody [EPR15036-16] - N-terminal (ab184159) at 1/50000 dilution

    Lane 1 : Jurkat lysate
    Lane 2 : K562 lysate
    Lane 3 : 293 lysate
    Lane 4 : HUVEC lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 34 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-TCEA1 antibody [EPR15036-16] - N-terminal (ab184159)
    Immunocytochemistry/ Immunofluorescence - Anti-TCEA1 antibody [EPR15036-16] - N-terminal (ab184159)

    Immunofluorscence analysis of HUVEC cells labeling TCEA1 with ab184159 at 1/250 dilution. Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution (left). Counter-stained with Dapi (right). 

  • Immunoprecipitation - Anti-TCEA1 antibody [EPR15036-16] - N-terminal (ab184159)
    Immunoprecipitation - Anti-TCEA1 antibody [EPR15036-16] - N-terminal (ab184159)

    Immunoprecipitation of Jurkat lysate labelling TCEA1 with ab184159 at 1/50 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody used at 1/1000 dilution

References

ab184159 has not yet been referenced specifically in any publications.

Publishing research using ab184159? Please let us know so that we can cite the reference in this datasheet.

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