RecombinantRabMAb

Recombinant Anti-TCEA1 antibody [EPR15036-16] - N-terminal (ab184159)

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Western blot - Anti-TCEA1 antibody [EPR15036-16] - N-terminal (ab184159)
  • Immunocytochemistry/ Immunofluorescence - Anti-TCEA1 antibody [EPR15036-16] - N-terminal (ab184159)
  • Immunoprecipitation - Anti-TCEA1 antibody [EPR15036-16] - N-terminal (ab184159)
  • Anti-TCEA1 antibody [EPR15036-16] - N-terminal (ab184159)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15036-16] to TCEA1 - N-terminal
  • Suitable for: WB, IP, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-TCEA1 antibody [EPR15036-16] - N-terminal
    See all TCEA1 primary antibodies

  • Description

    Rabbit monoclonal [EPR15036-16] to TCEA1 - N-terminal

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human TCEA1 aa 1-150 (N terminal). The exact sequence is proprietary.
    Database link: P23193

  • Positive control

    • Jurkat, K562, 293 and HUVEC lysates. HUVEC cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab184159 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 34, 38 kDa (predicted molecular weight: 34 kDa).
IP 1/60 - 1/80.
ICC/IF 1/250.

Target

  • Function

    Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.

  • Involvement in disease

    Note=A chromosomal aberration involving TCEA1 may be a cause of salivary gland pleiomorphic adenomas (PA) [181030]. Pleiomorphic adenomas are the most common benign epithelial tumors of the salivary gland. Translocation t(3;8)(p21;q12) with PLAG1.

  • Sequence similarities

    Belongs to the TFS-II family.
    Contains 1 TFIIS central domain.
    Contains 1 TFIIS N-terminal domain.
    Contains 1 TFIIS-type zinc finger.

  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • GTF2S antibody
    • SII antibody
    • TCEA antibody
    • Tcea1 antibody
    • TCEA1_HUMAN antibody
    • TF2S antibody
    • TFIIS antibody
    • Transcription elongation factor A (SII) 1 antibody
    • Transcription elongation factor A protein 1 antibody
    • Transcription elongation factor A, 1 antibody
    • Transcription elongation factor S-II protein 1 antibody
    • Transcription elongation factor TFIIS.o antibody
    see all

Images

  • Western blot - Anti-TCEA1 antibody [EPR15036-16] - N-terminal (ab184159)
    Western blot - Anti-TCEA1 antibody [EPR15036-16] - N-terminal (ab184159)
    All lanes : Anti-TCEA1 antibody [EPR15036-16] - N-terminal (ab184159) at 1/50000 dilution

    Lane 1 : Jurkat lysate
    Lane 2 : K562 lysate
    Lane 3 : 293 lysate
    Lane 4 : HUVEC lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 34 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-TCEA1 antibody [EPR15036-16] - N-terminal (ab184159)
    Immunocytochemistry/ Immunofluorescence - Anti-TCEA1 antibody [EPR15036-16] - N-terminal (ab184159)

    Immunofluorscence analysis of HUVEC cells labeling TCEA1 with ab184159 at 1/250 dilution. Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution (left). Counter-stained with Dapi (right). 

  • Immunoprecipitation - Anti-TCEA1 antibody [EPR15036-16] - N-terminal (ab184159)
    Immunoprecipitation - Anti-TCEA1 antibody [EPR15036-16] - N-terminal (ab184159)

    Immunoprecipitation of Jurkat lysate labelling TCEA1 with ab184159 at 1/50 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody used at 1/1000 dilution

  • Anti-TCEA1 antibody [EPR15036-16] - N-terminal (ab184159)
    Anti-TCEA1 antibody [EPR15036-16] - N-terminal (ab184159)

References (0)

Publishing research using ab184159? Please let us know so that we can cite the reference in this datasheet.

ab184159 has not yet been referenced specifically in any publications.

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