Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15036] to TCEA1
- Suitable for: WB, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
Product nameAnti-TCEA1 antibody [EPR15036]
See all TCEA1 primary antibodies
DescriptionRabbit monoclonal [EPR15036] to TCEA1
Tested applicationsSuitable for: WB, ICC/IF, IPmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment within Human TCEA1 aa 1-150. The exact sequence is proprietary.
Database link: P23193
- Jurkat, HEK293, K562 cell lysates; HUVEC cells
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab184158 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 38 kDa (predicted molecular weight: 34 kDa).|
|IP||1/60 - 1/80.|
FunctionNecessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.
Involvement in diseaseNote=A chromosomal aberration involving TCEA1 may be a cause of salivary gland pleiomorphic adenomas (PA) . Pleiomorphic adenomas are the most common benign epithelial tumors of the salivary gland. Translocation t(3;8)(p21;q12) with PLAG1.
Sequence similaritiesBelongs to the TFS-II family.
Contains 1 TFIIS central domain.
Contains 1 TFIIS N-terminal domain.
Contains 1 TFIIS-type zinc finger.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- GTF2S antibody
- SII antibody
- TCEA antibody
All lanes : Anti-TCEA1 antibody [EPR15036] (ab184158) at 1/50000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : 293 cell lysate
Lane 3 : K562 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 34 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
Immunofluorescence analysis of paraformaldehyde-fixed HUVEC cells, staining TCEA1 (red) with ab184158 at 1/250 dilution. Alexa Fluor®555-conjugated goat anti-rabbit IgG was used as a secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).
Western blot analysis on immunoprecipitation pellet from Jurkat cell lysate, labeling TCEA1 immunoprecipitated using ab184158 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
ab184158 has not yet been referenced specifically in any publications.