Key features and details
- Rabbit polyclonal to TCEB2/Elongin-B
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-TCEB2/Elongin-B antibody
See all TCEB2/Elongin-B primary antibodies
DescriptionRabbit polyclonal to TCEB2/Elongin-B
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rabbit, Goat, Cow, Dog, Pig, Chimpanzee, Lizard, Snake, Cynomolgus monkey, Rhesus monkey, Elephant
- Jurkat, 293T, HeLa, mouse TCMK-1 and mouse NIH3T3 whole cell lysates.
Previously labelled as TCEB2.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab168836 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2500. Predicted molecular weight: 13 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionSIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex).
The elongin BC complex seems to be involved as an adapter protein in the proteasomal degradation of target proteins via different E3 ubiquitin ligase complexes, including the von Hippel-Lindau ubiquitination complex CBC(VHL). By binding to BC-box motifs it seems to link target recruitment subunits, like VHL and members of the SOCS box family, to Cullin/RBX1 modules that activate E2 ubiquitination enzymes.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesContains 1 ubiquitin-like domain.
- Information by UniProt
- EloB antibody
- ELOB_HUMAN antibody
- Elongin 18 kDa subunit antibody
All lanes : Anti-TCEB2/Elongin-B antibody (ab168836) at 1 µg/ml
Lane 1 : Jurkat whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lane 5 : NIH3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 13 kDa
Exposure time: 3 minutes
Detection of TCEB2/Elongin-B in Immunoprecipitates of Jurkat whole cell lysate (1 mg for IP, 20% of IP loaded) using ab168836 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 3 minutes.
ab168836 has been referenced in 1 publication.
- Chuang TW et al. A Point Mutation in the Exon Junction Complex Factor Y14 Disrupts Its Function in mRNA Cap Binding and Translation Enhancement. J Biol Chem 291:8565-74 (2016). PubMed: 26887951