Recombinant Anti-TCEB2/Elongin-B antibody [EPR10440(B)] (ab151743)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10440(B)] to TCEB2/Elongin-B
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-TCEB2/Elongin-B antibody [EPR10440(B)]
See all TCEB2/Elongin-B primary antibodies -
Description
Rabbit monoclonal [EPR10440(B)] to TCEB2/Elongin-B -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human TCEB2/Elongin-B aa 1-100. The exact sequence is proprietary.
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Positive control
- Jurkat, HeLa, K562 and A431 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10440(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151743 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 13 kDa.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 13 kDa. |
Target
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Function
SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex).
The elongin BC complex seems to be involved as an adapter protein in the proteasomal degradation of target proteins via different E3 ubiquitin ligase complexes, including the von Hippel-Lindau ubiquitination complex CBC(VHL). By binding to BC-box motifs it seems to link target recruitment subunits, like VHL and members of the SOCS box family, to Cullin/RBX1 modules that activate E2 ubiquitination enzymes. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 1 ubiquitin-like domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6923 Human
- Omim: 600787 Human
- SwissProt: Q15370 Human
- Unigene: 172772 Human
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Alternative names
- EloB antibody
- ELOB_HUMAN antibody
- Elongin 18 kDa subunit antibody
see all
Images
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All lanes : Anti-TCEB2/Elongin-B antibody [EPR10440(B)] (ab151743) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : K562 cell lysate
Lane 4 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 13 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab151743 has been referenced in 1 publication.
- Zhang W et al. Tumor-associated antigen Prame targets tumor suppressor p14/ARF for degradation as the receptor protein of CRL2Prame complex. Cell Death Differ 28:1926-1940 (2021). PubMed: 33504946