Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10441(B)] to TCEB2/Elongin-B
- Suitable for: WB, Flow Cyt, IP
- Reacts with: Human
Product nameAnti-TCEB2/Elongin-B antibody [EPR10441(B)]
See all TCEB2/Elongin-B primary antibodies
DescriptionRabbit monoclonal [EPR10441(B)] to TCEB2/Elongin-B
Tested applicationsSuitable for: WB, Flow Cyt, IPmore details
Unsuitable for: ICC/IF or IHC-P
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human TCEB2/Elongin-B.
Database link: Q15370
- Jurkat, A431, K562 cell lysates; Jurkat cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Previously labelled as TCEB2.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab154854 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 13 kDa.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IP||1/10 - 1/100.|
FunctionSIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex).
The elongin BC complex seems to be involved as an adapter protein in the proteasomal degradation of target proteins via different E3 ubiquitin ligase complexes, including the von Hippel-Lindau ubiquitination complex CBC(VHL). By binding to BC-box motifs it seems to link target recruitment subunits, like VHL and members of the SOCS box family, to Cullin/RBX1 modules that activate E2 ubiquitination enzymes.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesContains 1 ubiquitin-like domain.
- Information by UniProt
- EloB antibody
- ELOB_HUMAN antibody
- Elongin 18 kDa subunit antibody
All lanes : Anti-TCEB2/Elongin-B antibody [EPR10441(B)] (ab154854) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : A431 cell lysate
Lane 3 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 13 kDa
Flow cytometric analysis of permeabilized Jurkat cells labeling TCEB2/Elongin-B with ab154854 at 1/10 dilution (red). Rabbit IgG negative control (green).
ab154854 has not yet been referenced specifically in any publications.