Key features and details
- Rabbit polyclonal to TCF-25
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-TCF-25 antibody
See all TCF-25 primary antibodies
DescriptionRabbit polyclonal to TCF-25
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow, Dog, Pig, Rhesus monkey, Gorilla, Chinese hamster, Xenopus tropicalis
Synthetic peptide within Human TCF-25 aa 1-50. The exact sequence is proprietary. (NP_055787.1).
Database link: Q9BQ70
- WB: HeLa, HEK-293T and NIH/3T3 whole cell lysates. IP: HeLa whole cell lysate.
Previously labelled as Transcription factor 25.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab225627 was affinity purified using an epitope specific to TCF-25 immobilized on solid support.
Our Abpromise guarantee covers the use of ab225627 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 77 kDa.|
|IP||Use a concentration of 2 - 5 µg/ml.|
FunctionMay play a role in cell death control. Acts as a transcriptional repressor. Has been shown to repress transcription of SRF in vitro and so may play a role in heart development.
Tissue specificityIn the embryo, widely expressed with highest levels in brain. In the adult, highest expression is found in the heart.
Sequence similaritiesBelongs to the TCF25 family.
DomainThe C-terminal region mediates transcriptional repression.
Cellular localizationNucleus. Some staining in the cytosol.
- Information by UniProt
- FKSG26 antibody
- hKIAA1049 antibody
- Hulp1 antibody
All lanes : Anti-TCF-25 antibody (ab225627) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 77 kDa
Exposure time: 3 minutes
TCF-25 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225627 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab225627 at 0.4 µg/ml.
Lane 1: ab225627 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab225627 has not yet been referenced specifically in any publications.