Overview

  • Product name

  • Description

    Rabbit polyclonal to TCF-25
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Dog, Pig, Rhesus monkey, Gorilla, Chinese hamster, Xenopus tropicalis
  • Immunogen

    Synthetic peptide within Human TCF-25 aa 1-50. The exact sequence is proprietary. (NP_055787.1).
    Database link: Q9BQ70

  • Positive control

    • WB: HeLa, HEK-293T and NIH/3T3 whole cell lysates. IP: HeLa whole cell lysate.
  • General notes

    Previously labelled as Transcription factor 25. 

Properties

Applications

Our Abpromise guarantee covers the use of ab225627 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 77 kDa.
IP Use a concentration of 2 - 5 µg/ml.

Target

Images

  • All lanes : Anti-TCF-25 antibody (ab225627) at 0.04 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
    Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
    Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 77 kDa


    Exposure time: 3 minutes
  • TCF-25 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225627 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab225627 at 0.4 µg/ml.

    Lane 1: ab225627 IP in HeLa whole cell lysate.

    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection:  Chemiluminescence with exposure time of 30 seconds.

References

ab225627 has not yet been referenced specifically in any publications.

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