Overview

  • Product name

  • Description

    Rabbit polyclonal to TCF-25
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Recombinant fragment corresponding to Human TCF-25 aa 427-648.
    Database link: NP_055787

  • Positive control

    • Hela whole cell lysate, cultured HeLa cells, FaDu xenograft. 293T and Molt-4 cell lines.
  • General notes

    Previously labelled as Transcription factor 25. 

Properties

Applications

Our Abpromise guarantee covers the use of ab96813 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 77 kDa.
IHC-P 1/100 - 1/250.
ICC/IF 1/100 - 1/200.

Target

  • Function

    May play a role in cell death control. Acts as a transcriptional repressor. Has been shown to repress transcription of SRF in vitro and so may play a role in heart development.
  • Tissue specificity

    In the embryo, widely expressed with highest levels in brain. In the adult, highest expression is found in the heart.
  • Sequence similarities

    Belongs to the TCF25 family.
  • Domain

    The C-terminal region mediates transcriptional repression.
  • Cellular localization

    Nucleus. Some staining in the cytosol.
  • Information by UniProt
  • Database links

  • Alternative names

    • FKSG26 antibody
    • hKIAA1049 antibody
    • Hulp1 antibody
    • KIAA1049 antibody
    • Nuclear localized protein 1 antibody
    • NULP1 antibody
    • PRO2620 antibody
    • TCF-25 antibody
    • Tcf25 antibody
    • TCF25_HUMAN antibody
    • Transcription factor 25 (basic helix loop helix) antibody
    • Transcription factor 25 antibody
    see all

Images

  • Anti-TCF-25 antibody (ab96813) at 1/500 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size: 77 kDa



    7.5% SDS Page

     

    Secondary antibody - anti-rabbit HRP (ab6721)

  • Immunohistochemical analysis of paraffin-embedded FaDu xenograft, using ab96813 antibody at 1/100 dilution.
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab96813 antibody at 1/200 dilution. Bottom picture is the same as the top one but is merged with fluorescent DNA probe.

References

ab96813 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cell line)
Gel Running Conditions
Reduced Denaturing
Loading amount
40 µg
Specification
HeLa cell line
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 20°C

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Verified customer

Submitted Sep 25 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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