Key features and details
- Rabbit polyclonal to TCF-25
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TCF-25 antibody
See all TCF-25 primary antibodies
DescriptionRabbit polyclonal to TCF-25
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Cow
Recombinant fragment corresponding to Human TCF-25 aa 427-648.
Database link: NP_055787
- Hela whole cell lysate, cultured HeLa cells, FaDu xenograft. 293T and Molt-4 cell lines.
Previously labelled as Transcription factor 25.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab96813 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 77 kDa.|
|IHC-P||1/100 - 1/250.|
|ICC/IF||1/100 - 1/200.|
FunctionMay play a role in cell death control. Acts as a transcriptional repressor. Has been shown to repress transcription of SRF in vitro and so may play a role in heart development.
Tissue specificityIn the embryo, widely expressed with highest levels in brain. In the adult, highest expression is found in the heart.
Sequence similaritiesBelongs to the TCF25 family.
DomainThe C-terminal region mediates transcriptional repression.
Cellular localizationNucleus. Some staining in the cytosol.
- Information by UniProt
- FKSG26 antibody
- hKIAA1049 antibody
- Hulp1 antibody
Immunohistochemical analysis of paraffin-embedded FaDu xenograft, using ab96813 antibody at 1/100 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab96813 antibody at 1/200 dilution. Bottom picture is the same as the top one but is merged with fluorescent DNA probe.
ab96813 has not yet been referenced specifically in any publications.