Overview

  • Product name

  • Description

    Rabbit polyclonal to TCF-4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Horse, Cow, Dog, Macaque monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Mouse TCF-4 aa 50-150 conjugated to keyhole limpet haemocyanin.
    Database link: Q60722
    (Peptide available as ab229703)

  • Positive control

    • This antibody gave a positive signal in the following Mouse tissue lysates: Cortex; Cerebellum; Olfactory Bulb; Hippocampus; Liver; as well as the following whole cell lysates: HepG2; HCT116; Caco2.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab130014 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa).Can be blocked with Mouse TCF-4 peptide (ab229703).

Target

  • Function

    Transcription factor that binds to the immunoglobulin enchancer Mu-E5/KE5-motif. Binds to the E-box present in the somatostatin receptor 2 initiator element (SSTR2-INR) to activate transcription (By similarity). Preferentially binds to either 5'-ACANNTGT-3' or 5'-CCANNTGG-3'.
  • Tissue specificity

    Expressed in adult heart, brain, placenta, skeletal muscle and to a lesser extent in the lung. In developing embryonic tissues, expression mostly occurs in the brain.
  • Involvement in disease

    Defects in TCF4 are a cause of Pitt-Hopkins syndrome (PTHS) [MIM:610954]. PTHS is a rare syndromic encephalopathy characterized by severe psychomotor delay, epilepsy, daily bouts of diurnal hyperventilation starting in infancy, mild postnatal growth retardation, postnatal microcephaly, and distinctive facial features. Since most hitherto reported cases have been sporadic, with males and females equally affected, PTHS is regarded as an autosomal dominant condition.
  • Sequence similarities

    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Domain

    the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • bHLHb19 antibody
    • Class B basic helix-loop-helix protein 19 antibody
    • E2 2 antibody
    • FECD3 antibody
    • Immunoglobulin transcription factor 2 antibody
    • ITF 2 antibody
    • ITF-2 antibody
    • ITF2 antibody
    • ITF2_HUMAN antibody
    • MGC149723 antibody
    • MGC149724 antibody
    • PTHS antibody
    • SEF 2 antibody
    • SEF-2 antibody
    • SEF2 1 antibody
    • SEF2 1A antibody
    • SEF2 1B antibody
    • SEF2 1D antibody
    • SEF2 antibody
    • SL3 3 enhancer factor 2 antibody
    • SL3-3 enhancer factor 2 antibody
    • TCF 4 antibody
    • TCF-4 antibody
    • TCF4 antibody
    • Transcription factor 4 antibody
    • Transcription factor 4, isoform C antibody
    • Transcription factor 4, isoform D antibody
    • Transcription factor 4, isoform E antibody
    • Transcription factor 4, isoform L antibody
    • Transcription factor 4, isoform M antibody
    • Transcription factor 4, isoform R antibody
    see all

Images

  • All lanes : Anti-TCF-4 antibody (ab130014) at 1 µg/ml

    Lane 1 : Mouse Cortex Tissue Lysate
    Lane 2 : Cerebellum Mouse Tissue Lysate
    Lane 3 : Mouse Olfactory Bulb Lysate
    Lane 4 : Mouse Hippocampus Tissue Lysate
    Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 6 : Liver (Mouse) Tissue Lysate
    Lane 7 : HCT 116 (Human Colorectal Carcinoma) Whole Cell Lysate
    Lane 8 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 71 kDa
    Observed band size: 71 kDa
    Additional bands at: 260 kDa, 45 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 2 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab130014 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

This product has been referenced in:

  • Jung M  et al. Analysis of the expression pattern of the schizophrenia-risk and intellectual disability gene TCF4 in the developing and adult brain suggests a role in development and plasticity of cortical and hippocampal neurons. Mol Autism 9:20 (2018). Read more (PubMed: 29588831) »
  • Rathert P  et al. Transcriptional plasticity promotes primary and acquired resistance to BET inhibition. Nature 525:543-7 (2015). Read more (PubMed: 26367798) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Cortex P7)
Antigen retrieval step
None
Permeabilization
Yes - Triton
Specification
Cortex P7
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C
Fixative
Paraformaldehyde

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Verified customer

Submitted Aug 23 2019

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