Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [7B3] to TCF12
- Suitable for: WB
- Reacts with: Mouse, Human
Product nameAnti-TCF12 antibody [7B3]
See all TCF12 primary antibodies
DescriptionRat monoclonal [7B3] to TCF12
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P
Species reactivityReacts with: Mouse, Human
Recombinant fragment. This information is considered to be commercially sensitive.
- WB: SW620, HeLa, Raji, Jurkat and WEHI-231 cell lysate.
This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact firstname.lastname@example.org.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab243149 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.394 µg/ml. Predicted molecular weight: 72 kDa.|
FunctionBinds specifically to oligomers of E-box motifs. May play important roles during development of the nervous system as well as in other organ systems.
Tissue specificityExpressed in several tissues and cell types including skeletal muscle, thymus, and a B-cell line.
Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
Domainthe 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- bHLHb20 antibody
- Class B basic helix-loop-helix protein 20 antibody
- DNA binding protein HTF4 antibody
All lanes : Anti-TCF12 antibody [7B3] (ab243149) at 0.394 µg/ml
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : SW620 (human colorectal adenocarcinoma epithelial cell), whole cell lysate
Lane 3 : Raji (human Burkitt’s lymphoma B lymphocyte), whole cell lysate
Lane 4 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
Lane 5 : WEHI-231 (mouse B cell lymphoma B lymphocyte), whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution
Predicted band size: 72 kDa
Observed band size: 92 kDa why is the actual band size different from the predicted?
The expression molecular weight observed is consistent with what has been described in the literature (PMID: 16785505).
Blocking/diluting buffer and concentration: 5% NFDM/TBST.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab243149 has not yet been referenced specifically in any publications.