Recombinant Anti-TCF12 antibody [7B3] - BSA and Azide free (ab252269)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [7B3] to TCF12 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-TCF12 antibody [7B3] - BSA and Azide free
See all TCF12 primary antibodies -
Description
Rat monoclonal [7B3] to TCF12 - BSA and Azide free -
Host species
Rat -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: SW620, HeLa, Raji, Jurkat and WEHI-231 cell lysate.
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General notes
ab252269 is the carrier-free version of ab243149.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
7B3 -
Isotype
IgG2b -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab252269 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.394 µg/ml. Predicted molecular weight: 72 kDa.
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Notes |
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WB
Use a concentration of 0.394 µg/ml. Predicted molecular weight: 72 kDa. |
Target
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Function
Binds specifically to oligomers of E-box motifs. May play important roles during development of the nervous system as well as in other organ systems. -
Tissue specificity
Expressed in several tissues and cell types including skeletal muscle, thymus, and a B-cell line. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain. -
Domain
the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6938 Human
- Entrez Gene: 21406 Mouse
- Omim: 600480 Human
- SwissProt: Q99081 Human
- SwissProt: Q61286 Mouse
- Unigene: 511504 Human
- Unigene: 171615 Mouse
- Unigene: 480117 Mouse
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Alternative names
- bHLHb20 antibody
- Class B basic helix-loop-helix protein 20 antibody
- DNA binding protein HTF4 antibody
see all
Images
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All lanes : Anti-TCF12 antibody [7B3] (ab243149) at 0.394 µg/ml
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : SW620 (human colorectal adenocarcinoma epithelial cell), whole cell lysate
Lane 3 : Raji (human Burkitt’s lymphoma B lymphocyte), whole cell lysate
Lane 4 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
Lane 5 : WEHI-231 (mouse B cell lymphoma B lymphocyte), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution
Predicted band size: 72 kDaThe expression molecular weight observed is consistent with what has been described in the literature (PMID: 16785505).
Blocking/diluting buffer and concentration: 5% NFDM/TBST
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243149).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab252269 has not yet been referenced specifically in any publications.