Key features and details
- Rabbit polyclonal to TCF12
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TCF12 antibody
See all TCF12 primary antibodies
DescriptionRabbit polyclonal to TCF12
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human TCF12 aa 50-100. The exact sequence is proprietary. NP_996919.1
Database link: Q99081
- WB: HeLa whole cell lysate. IP: HeLa whole cell lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245540 was affinity purified using an epitope specific to TCF12 immobilized on solid support.
Our Abpromise guarantee covers the use of ab245540 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 73 kDa.|
FunctionBinds specifically to oligomers of E-box motifs. May play important roles during development of the nervous system as well as in other organ systems.
Tissue specificityExpressed in several tissues and cell types including skeletal muscle, thymus, and a B-cell line.
Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
Domainthe 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- bHLHb20 antibody
- Class B basic helix-loop-helix protein 20 antibody
- DNA binding protein HTF4 antibody
All lanes : Anti-TCF12 antibody (ab245540) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 73 kDa
Exposure time: 3 minutes
TCF12 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245540 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245540 at 1 µg/ml.
Lane 1: ab245540 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab245540 has not yet been referenced specifically in any publications.