Key features and details
- Rabbit polyclonal to TCF12
- Suitable for: IP, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TCF12 antibody
See all TCF12 primary antibodies
DescriptionRabbit polyclonal to TCF12
Tested applicationsSuitable for: IP, IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Chimpanzee, Lizard, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
Synthetic peptide corresponding to a region between residue 1 and 50 of human TCF12 (NP_996919.1).
- Whole cell lysate from HeLa cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab70746 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|WB||1/2000 - 1/10000. Detects a band of approximately 85 kDa (predicted molecular weight: 73 kDa).|
FunctionBinds specifically to oligomers of E-box motifs. May play important roles during development of the nervous system as well as in other organ systems.
Tissue specificityExpressed in several tissues and cell types including skeletal muscle, thymus, and a B-cell line.
Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
Domainthe 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- bHLHb20 antibody
- Class B basic helix-loop-helix protein 20 antibody
- DNA binding protein HTF4 antibody
All lanes : Anti-TCF12 antibody (ab70746) at 1/10000 dilution
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 73 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa, 120 kDa, 190 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling TCF12 with ab70746 at 1/1000 (1µg/ml). Detection: DAB.
1mg whole cell lysate from HeLa cells was immunprecipitated with ab70746 at 3µg/mg lysate. For the subsequent western blot, 20% of the immunoprecipitate was loaded per lane, and probed with ab707345 at 1ug/ml. Detection: chemiluminescence with exposure time of 10 seconds
ab70746 has not yet been referenced specifically in any publications.