Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab32981 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ChIP Use at an assay dependent concentration. PubMed: 23112163

   5 µg used per ChIP

ELISA 1/12500.
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 20 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Images

  • Immunohistochemistry of Human Brain, cortex, senile plaque tissue at an antibody concentration of 5.0µg/ml using anti-TCF21 antibody (ab32981)

  • Anti-TCF21 antibody - ChIP Grade (ab32981) at 0.5 µg/ml + fetal lung lysate at 10 µg

    Secondary
    HRP conjugated anti Rabbit IgG at 1/50000 dilution

    Predicted band size: 20 kDa
    Observed band size: 17 kDa
    why is the actual band size different from the predicted?

References

This product has been referenced in:
  • Gooskens SL  et al. TCF21 hypermethylation regulates renal tumor cell clonogenic proliferation and migration. Mol Oncol 12:166-179 (2018). WB ; Human . Read more (PubMed: 29080283) »
  • Wu PL  et al. Transcription factor 21 regulates expression of ERß and SF-1 via upstream stimulatory factor-2 in endometriotic tissues. Biochim Biophys Acta Gene Regul Mech 1861:706-717 (2018). Read more (PubMed: 30018006) »
See all 8 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Heart)
Permeabilization
Yes - triton for PFA fixed
Specification
Heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 30°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jul 09 2018

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (squamous cell carcinoma, tongue)
Specification
squamous cell carcinoma, tongue
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Jul 20 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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