Recombinant Anti-TCF21 antibody [EPR13449] (ab182134)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13449] to TCF21
- Suitable for: WB, IP, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-TCF21 antibody [EPR13449]
See all TCF21 primary antibodies -
Description
Rabbit monoclonal [EPR13449] to TCF21 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa and 293 cell lysates; HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13449 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab182134 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 26 kDa (predicted molecular weight: 20 kDa).
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IP | (1) |
1/70.
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Flow Cyt (Intra) |
1/130.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 26 kDa (predicted molecular weight: 20 kDa). |
IP
1/70. |
Flow Cyt (Intra)
1/130. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Involved in epithelial-mesenchymal interactions in kidney and lung morphogenesis that include epithelial differentiation and branching morphogenesis. May play a role in the specification or differentiation of one or more subsets of epicardial cell types. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6943 Human
- Omim: 603306 Human
- SwissProt: O43680 Human
- Unigene: 78061 Human
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Alternative names
- bHLHa23 antibody
- Capsulin antibody
- Class A basic helix-loop-helix protein 23 antibody
see all
Images
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All lanes : Anti-TCF21 antibody [EPR13449] (ab182134) at 1/5000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 20 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted? -
Intracellular flow cytometric analysis of 2% paraformaldehyde-fixedHeLa cells labeling TCF21with ab182134 at 1/130 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
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Western blot analysis of immunoprecipitation pellet from 293 cell lysate immunoprecipitated using ab182134 at 1/70 dilution (Lane 1). Lane 2: Negative control.
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab182134 has been referenced in 2 publications.
- Finer G et al. Stromal Transcription Factor 21 Regulates Development of the Renal Stroma via Interaction with Wnt/β-Catenin Signaling. Kidney360 3:1228-1241 (2022). PubMed: 35919523
- Zhu H et al. TCF21 regulates miR-10a-5p/LIN28B signaling to block the proliferation and invasion of melanoma cells. PLoS One 16:e0255971 (2021). PubMed: 34424910