Overview

  • Product name

    Anti-TCF3 / E2A antibody - N-terminal
    See all TCF3 / E2A primary antibodies
  • Description

    Rabbit polyclonal to TCF3 / E2A - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human TCF3/ E2A (N terminal). The exact sequence is proprietary.
    Database link: P15923

  • Positive control

    • WB: Jurkat, Raji, and NCI-H929 whole cell extracts; NCI-H929 nuclear extract. IP: Jurkat whole cell extract. ICC/IF: MCF7 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab229605 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/3000. Predicted molecular weight: 68 kDa.
IP 1/100 - 1/500.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Dimers bind DNA on E-box motifs: 5'-CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer.
  • Involvement in disease

    Note=Chromosomal aberrations involving TCF3 are cause of forms of pre-B-cell acute lymphoblastic leukemia (B-ALL). Translocation t(1;19)(q23;p13.3) with PBX1; Translocation t(17;19)(q22;p13.3) with HLF. Inversion inv(19)(p13;q13) with TFPT.
  • Sequence similarities

    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Domain

    the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
  • Post-translational
    modifications

    Phosphorylated following NGF stimulation.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • AGM8 antibody
    • bHLHb21 antibody
    • Class B basic helix-loop-helix protein 21 antibody
    • E12 antibody
    • E2A antibody
    • E2A immunoglobulin enhancer binding factors E12/E47 antibody
    • E47 antibody
    • Helix loop helix protein HE47 antibody
    • Immunoglobulin enhancer-binding factor E12/E47 antibody
    • Immunoglobulin transcription factor 1 antibody
    • ITF1 antibody
    • Kappa-E2-binding factor antibody
    • MGC129647 antibody
    • MGC129648 antibody
    • Negative vitamin D response element binding protein antibody
    • NOL1-TCF3 fusion antibody
    • TCF-3 antibody
    • Tcf3 antibody
    • TFE2_HUMAN antibody
    • transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47) antibody
    • Transcription factor 3 antibody
    • transcription factor 3 variant 3 antibody
    • Transcription factor E2-alpha antibody
    • Transcription factor ITF-1 antibody
    • VDIR antibody
    • VDR interacting repressor antibody
    • vitamin D receptor-interacting repressor antibody
    see all

Images

  • All lanes : Anti-TCF3 / E2A antibody - N-terminal (ab229605) at 1/2500 dilution

    Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract
    Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell extract
    Lane 3 : NCI-H929 whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 68 kDa



    7.5% SDS-PAGE gel.

  • 4% paraformaldehyde-fixed MCF7 (human breast adenocarcinoma cell line) cells stained for TCF3 / E2A (green) using ab229605  at 1/500 dilution in ICC/IF.

    Nuclear counterstain: Hoechst 33342 (blue).

  • All lanes : Anti-TCF3 / E2A antibody - N-terminal (ab229605) at 1/3000 dilution

    Lane 1 : NCI-H929 whole cell extract
    Lane 2 : NCI-H929 nuclear extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 68 kDa



    7.5% SDS-PAGE gel.

  • TCF3 / E2A was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate with 4 μg ab229605. Western blot was performed from the immunoprecipitate using ab229605 at 1/500 dilution.

    Lane 1: Jurkat whole cell extract 50 μg (Input). 

    Lane 2:  4 μg of preimmune Rabbit IgG instead of ab229605 in Jurkat whole cell extract. 

    Lane 3: ab229605 IP in Jurkat whole cell extract.

References

ab229605 has not yet been referenced specifically in any publications.

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