Product nameAnti-TCF3 / E2A (phospho T355) antibody
See all TCF3 / E2A primary antibodies
DescriptionRabbit polyclonal to TCF3 / E2A (phospho T355)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Hamster, Dog
Synthetic peptide corresponding to Human TCF3/ E2A aa 350-450 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in both HeLa and HepG2 whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab85772 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 68 kDa).|
FunctionHeterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Dimers bind DNA on E-box motifs: 5'-CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer.
Involvement in diseaseNote=Chromosomal aberrations involving TCF3 are cause of forms of pre-B-cell acute lymphoblastic leukemia (B-ALL). Translocation t(1;19)(q23;p13.3) with PBX1; Translocation t(17;19)(q22;p13.3) with HLF. Inversion inv(19)(p13;q13) with TFPT.
Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
Domainthe 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
modificationsPhosphorylated following NGF stimulation.
- Information by UniProt
- AGM8 antibody
- bHLHb21 antibody
- Class B basic helix-loop-helix protein 21 antibody
All lanes : Anti-TCF3 / E2A (phospho T355) antibody (ab85772) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human TCF3 / E2A peptide (ab118180) at 1 µg/ml
Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate with Human TCF3 / E2A peptide (ab118180) at 1 µg/ml
Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human TCF3 / E2A (phospho T355) peptide (ab103576) at 1 µg/ml
Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate with Human TCF3 / E2A (phospho T355) peptide (ab103576) at 1 µg/ml
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 68 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
ab85772 has not yet been referenced specifically in any publications.