Product nameAnti-TCF7 antibody
See all TCF7 primary antibodies
DescriptionRabbit polyclonal to TCF7
Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
- WB: fetal thymus lysate. IHC-P: human kidney tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis antibody was purified by peptide affinity chromatography method.
Our Abpromise guarantee covers the use of ab30961 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.125 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 42 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use a concentration of 4 - 8 µg/ml.|
FunctionTranscriptional activator involved in T-cell lymphocyte differentiation. Necessary for the survival of CD4(+) CD8(+) immature thymocytes. Isoforms lacking the N-terminal CTNNB1 binding domain cannot fulfill this role. Binds to the T-lymphocyte-specific enhancer element (5'-WWCAAAG-3') found in the promoter of the CD3E gene. May also act as feedback transcriptional repressor of CTNNB1 and TCF7L2 target genes. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7 and CTNNB1.
Tissue specificityPredominantly in T-cells. Also detected in proliferating intestinal epithelial cells and in the basal epithelial cells of mammary gland epithelium.
Sequence similaritiesBelongs to the TCF/LEF family.
Contains 1 HMG box DNA-binding domain.
- Information by UniProt
- FLJ36364 antibody
- MGC47735 antibody
- OTTHUMP00000159391 antibody
Anti-TCF7 antibody (ab30961) at 0.125 µg/ml + fetal thymus lysate at 10 µg/ml
HRP conjugated anti rabbit IgG at a dilution of 1/ 50,000 - 1/100,000
Developed using the ECL technique.
Predicted band size: 42 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
Additional bands at: 25 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed parraffin-embedded sections) analysis of human kidney tissue labelling Transcription factor 7 with ab30961 at 4-8µg/ml. Positively labelled cells (epithelial cells of renal tubule) are indicated by arrows. Magnification: 400X.
This product has been referenced in:
- Yu F et al. Evaluation of expression of the Wnt signaling components in canine mammary tumors via RT2Profiler PCR Array and immunochemistry assays. J Vet Sci 18:359-367 (2017). Read more (PubMed: 27586466) »
- Li W et al. Genome-wide analyses identify KLF4 as an important negative regulator in T-cell acute lymphoblastic leukemia through directly inhibiting T-cell associated genes. Mol Cancer 14:26 (2015). Read more (PubMed: 25644173) »