The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.125 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 42 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use a concentration of 4 - 8 µg/ml.
Transcriptional activator involved in T-cell lymphocyte differentiation. Necessary for the survival of CD4(+) CD8(+) immature thymocytes. Isoforms lacking the N-terminal CTNNB1 binding domain cannot fulfill this role. Binds to the T-lymphocyte-specific enhancer element (5'-WWCAAAG-3') found in the promoter of the CD3E gene. May also act as feedback transcriptional repressor of CTNNB1 and TCF7L2 target genes. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7 and CTNNB1.
Predominantly in T-cells. Also detected in proliferating intestinal epithelial cells and in the basal epithelial cells of mammary gland epithelium.
Belongs to the TCF/LEF family. Contains 1 HMG box DNA-binding domain.
Immunohistochemistry (Formalin/PFA-fixed parraffin-embedded sections) analysis of human kidney tissue labelling Transcription factor 7 with ab30961 at 4-8µg/ml. Positively labelled cells (epithelial cells of renal tubule) are indicated by arrows. Magnification: 400X.
Yu F et al. Evaluation of expression of the Wnt signaling components in canine mammary tumors via RT2Profiler PCR Array and immunochemistry assays. J Vet Sci18:359-367 (2017).
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Li W et al. Genome-wide analyses identify KLF4 as an important negative regulator in T-cell acute lymphoblastic leukemia through directly inhibiting T-cell associated genes. Mol Cancer14:26 (2015).
Read more (PubMed: 25644173) »