Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TCF7 antibody

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Intestine)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization
No
Specification
Intestine
Blocking step
Normal horse serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
Formaldehyde

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: CAS Block
Dilution
1/500

Secondary antibody

Name
Non-Abcam antibody was used: Anti-Rabbit IgG
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Additional data

Additional Notes
Specific staining was detected in lymph nodes in the gut. Gut epithelium was not stained as predicted. Picture was taken in 20x magnification.

Eliran Kadosh

Verified customer

Submitted May 15 2019

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