Overview

  • Product name

    Anti-TCF7 antibody [EPR2035]
    See all TCF7 primary antibodies
  • Description

    Rabbit monoclonal [EPR2035] to TCF7
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human TCF7 aa 100-200. The exact sequence is proprietary.
    Database link: P36402

  • Positive control

    • WB: Human thymus lysate
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab134127 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 25-55 kDa (predicted molecular weight: 42 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function

      Transcriptional activator involved in T-cell lymphocyte differentiation. Necessary for the survival of CD4(+) CD8(+) immature thymocytes. Isoforms lacking the N-terminal CTNNB1 binding domain cannot fulfill this role. Binds to the T-lymphocyte-specific enhancer element (5'-WWCAAAG-3') found in the promoter of the CD3E gene. May also act as feedback transcriptional repressor of CTNNB1 and TCF7L2 target genes. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7 and CTNNB1.
    • Tissue specificity

      Predominantly in T-cells. Also detected in proliferating intestinal epithelial cells and in the basal epithelial cells of mammary gland epithelium.
    • Sequence similarities

      Belongs to the TCF/LEF family.
      Contains 1 HMG box DNA-binding domain.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • FLJ36364 antibody
      • MGC47735 antibody
      • OTTHUMP00000159391 antibody
      • T cell factor 1 antibody
      • T cell specific transcription factor 1 antibody
      • T-cell factor 1 antibody
      • T-cell-specific transcription factor 1 antibody
      • TCF-1 antibody
      • TCF-7 antibody
      • TCF1 antibody
      • Tcf7 antibody
      • TCF7 transcription factor 7 antibody
      • TCF7_HUMAN antibody
      • Transcription factor 7 (T cell specific HMG box) antibody
      • Transcription factor 7 antibody
      • Transcription factor-7, T-cell specific antibody
      • Transcription factor-7, T-cell specific, 1 antibody
      see all

    Images

    • Anti-TCF7 antibody [EPR2035] (ab134127) at 1.2 µg/ml (purified) + Human thymus lysates at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 42 kDa



      Blocking and diluting buffer: 5% NFDM/TBST.

      This antibody recognizes multiple TCF7 isoforms reported by many papers (PMID: 20980261, 16424171, 24551047)

    References

    ab134127 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab134127.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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