Product nameAnti-TCF7 antibody [EPR2035] - BSA and Azide free
See all TCF7 primary antibodies
DescriptionRabbit monoclonal [EPR2035] to TCF7 - BSA and Azide free
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt or ICC/IF
Species reactivityReacts with: Human
Synthetic peptide within Human TCF7 aa 100-200. The exact sequence is proprietary.
Database link: P36402
- Jurkat nuclear extract lysate (ab14844) can be used as a positive control in WB.
ab236035 is the carrier-free version of ab134127 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab236035 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab236035 in the following tested applications.
|WB||Use at an assay dependent concentration. Detects a band of approximately 25-55 kDa (predicted molecular weight: 42 kDa).|
FunctionTranscriptional activator involved in T-cell lymphocyte differentiation. Necessary for the survival of CD4(+) CD8(+) immature thymocytes. Isoforms lacking the N-terminal CTNNB1 binding domain cannot fulfill this role. Binds to the T-lymphocyte-specific enhancer element (5'-WWCAAAG-3') found in the promoter of the CD3E gene. May also act as feedback transcriptional repressor of CTNNB1 and TCF7L2 target genes. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7 and CTNNB1.
Tissue specificityPredominantly in T-cells. Also detected in proliferating intestinal epithelial cells and in the basal epithelial cells of mammary gland epithelium.
Sequence similaritiesBelongs to the TCF/LEF family.
Contains 1 HMG box DNA-binding domain.
- Information by UniProt
- FLJ36364 antibody
- MGC47735 antibody
- OTTHUMP00000159391 antibody
Anti-TCF7 antibody [EPR2035] (ab134127) at 1.2 µg/ml + Human thymus lysates at 20 µg
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 42 kDa
Blocking and diluting buffer: 5% NFDM/TBST.
This antibody recognizes multiple TCF7 isoforms reported by many papers (PMID: 20980261, 16424171, 24551047)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134127).
ab236035 has not yet been referenced specifically in any publications.