Recombinant
RabMAb

Recombinant Anti-TCF7 antibody [EPR2035] - BSA and Azide free (ab236035)

Overview

  • Product name

    Anti-TCF7 antibody [EPR2035] - BSA and Azide free
    See all TCF7 primary antibodies
  • Description

    Rabbit monoclonal [EPR2035] to TCF7 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human TCF7 aa 100-200. The exact sequence is proprietary.
    Database link: P36402

  • Positive control

    • Jurkat nuclear extract lysate (ab14844) can be used as a positive control in WB.
  • General notes

    ab236035 is the carrier-free version of ab134127 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab236035 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab236035 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 25-55 kDa (predicted molecular weight: 42 kDa).
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function

      Transcriptional activator involved in T-cell lymphocyte differentiation. Necessary for the survival of CD4(+) CD8(+) immature thymocytes. Isoforms lacking the N-terminal CTNNB1 binding domain cannot fulfill this role. Binds to the T-lymphocyte-specific enhancer element (5'-WWCAAAG-3') found in the promoter of the CD3E gene. May also act as feedback transcriptional repressor of CTNNB1 and TCF7L2 target genes. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7 and CTNNB1.
    • Tissue specificity

      Predominantly in T-cells. Also detected in proliferating intestinal epithelial cells and in the basal epithelial cells of mammary gland epithelium.
    • Sequence similarities

      Belongs to the TCF/LEF family.
      Contains 1 HMG box DNA-binding domain.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • FLJ36364 antibody
      • MGC47735 antibody
      • OTTHUMP00000159391 antibody
      • T cell factor 1 antibody
      • T cell specific transcription factor 1 antibody
      • T-cell factor 1 antibody
      • T-cell-specific transcription factor 1 antibody
      • TCF-1 antibody
      • TCF-7 antibody
      • TCF1 antibody
      • Tcf7 antibody
      • TCF7 transcription factor 7 antibody
      • TCF7_HUMAN antibody
      • Transcription factor 7 (T cell specific HMG box) antibody
      • Transcription factor 7 antibody
      • Transcription factor-7, T-cell specific antibody
      • Transcription factor-7, T-cell specific, 1 antibody
      see all

    Images

    • Anti-TCF7 antibody [EPR2035] (ab134127) at 1.2 µg/ml + Human thymus lysates at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 42 kDa



      Blocking and diluting buffer: 5% NFDM/TBST.

      This antibody recognizes multiple TCF7 isoforms reported by many papers (PMID: 20980261, 16424171, 24551047)

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134127).

    References

    ab236035 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab236035.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up