Key features and details
- Rabbit polyclonal to TCF7L1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-TCF7L1 antibody
See all TCF7L1 primary antibodies
DescriptionRabbit polyclonal to TCF7L1
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Guinea pig, Cow, Dog
Synthetic peptide, corresponding to a region within N terminal amino acids 36-85 (DELIPFQDEG GEEQEPSSDS ASAQRDLDEV KSSLVNESEN QSSSSDSEAE) of Human TCF7L1, NP_112573
- 293T cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: PBS, 2% Sucrose
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab86175 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 63 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
FunctionParticipates in the Wnt signaling pathway. Binds to DNA and acts as a repressor in the absence of CTNNB1, and as an activator in its presence. Necessary for the terminal differentiation of epidermal cells, the formation of keratohyalin granules and the development of the barrier function of the epidermis (By similarity). Down-regulates NQO1, leading to increased mitomycin c resistance.
Tissue specificityDetected in hair follicles and skin keratinocytes, and at lower levels in stomach epithelium.
Sequence similaritiesBelongs to the TCF/LEF family.
Contains 1 HMG box DNA-binding domain.
DomainThe putative Groucho interaction domain between the N-terminal CTNNB1 binding domain and the HMG-box is necessary for repression of the transactivation mediated by TCF7L1 and CTNNB1.
- Information by UniProt
- bHLHb21 antibody
- HMG box transcription factor 3 antibody
- HMG box transcription factor antibody
Anti-TCF7L1 antibody (ab86175) at 1 µg/ml (5% skim milk/ PBS buffer) + 293T cell lysate at 10 µg with gel concentration: 12%
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 63 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
Additional bands at: >90 kDa, 38 kDa, 46 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin tissue labelling TCF7L1 with ab86175 at 5.0µg/ml.
ab86175 has been referenced in 1 publication.
- Murphy M et al. TCF7L1 Modulates Colorectal Cancer Growth by Inhibiting Expression of the Tumor-Suppressor Gene EPHB3. Sci Rep 6:28299 (2016). IHC . PubMed: 27333864