Overview

  • Product name

    Anti-TCF7L1 antibody [EPR2031]
    See all TCF7L1 primary antibodies
  • Description

    Rabbit monoclonal [EPR2031] to TCF7L1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human TCF7L1 (UniProt Q9HCS4).

  • Positive control

    • A431 and Human fetal small intestine lysates
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133360 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 63 kDa.
Flow Cyt 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function

      Participates in the Wnt signaling pathway. Binds to DNA and acts as a repressor in the absence of CTNNB1, and as an activator in its presence. Necessary for the terminal differentiation of epidermal cells, the formation of keratohyalin granules and the development of the barrier function of the epidermis (By similarity). Down-regulates NQO1, leading to increased mitomycin c resistance.
    • Tissue specificity

      Detected in hair follicles and skin keratinocytes, and at lower levels in stomach epithelium.
    • Sequence similarities

      Belongs to the TCF/LEF family.
      Contains 1 HMG box DNA-binding domain.
    • Domain

      The putative Groucho interaction domain between the N-terminal CTNNB1 binding domain and the HMG-box is necessary for repression of the transactivation mediated by TCF7L1 and CTNNB1.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • bHLHb21 antibody
      • HMG box transcription factor 3 antibody
      • HMG box transcription factor antibody
      • OTTMUSP00000023419 antibody
      • T cell factor 3 antibody
      • TCF 3 antibody
      • TCF-3 antibody
      • TCF3 antibody
      • Tcf7l1 antibody
      • TF7L1_HUMAN antibody
      • Transcription factor 7 like 1 (T-cell specific, HMG-box) antibody
      • Transcription factor 7 like 1 antibody
      • Transcription factor 7-like 1 antibody
      see all

    Images

    • All lanes : Anti-TCF7L1 antibody [EPR2031] (ab133360) at 1/1000 dilution

      Lane 1 : A431 lysate
      Lane 2 : Human fetal small intestine lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 63 kDa

    References

    This product has been referenced in:

    • Li FF  et al. Different effection of p.1125Val>Ala and rs11954856 in APC on Wnt signaling pathway. Oncotarget 8:70854-70864 (2017). Read more (PubMed: 29050326) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab133360.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up