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    tcf7l1-antibody-epr2031-bsa-and-azide-free-ab248495.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families HMG Box
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RecombinantRabMAb

Recombinant Anti-TCF7L1 antibody [EPR2031] - BSA and Azide free (ab248495)

  • Datasheet
  • Certificate of Compliance
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Western blot - Anti-TCF7L1 antibody [EPR2031] - BSA and Azide free (ab248495)
  • Anti-TCF7L1 antibody [EPR2031] - BSA and Azide free (ab248495)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2031] to TCF7L1 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Human

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Conjugation
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PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)

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Overview

  • Product name

    Anti-TCF7L1 antibody [EPR2031] - BSA and Azide free
    See all TCF7L1 primary antibodies
  • Description

    Rabbit monoclonal [EPR2031] to TCF7L1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human TCF7L1 aa 50-150. The exact sequence is proprietary.
    Database link: Q9HCS4

  • General notes

    ab248495 is the carrier-free version of ab133360 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab248495 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR2031
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HMG Box
    • Stem Cells
    • Signaling Pathways
    • Wnt
    • Nuclear
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding

Associated products

  • Alternative Versions

    • Anti-TCF7L1 antibody [EPR2031] (ab133360)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)

Applications

Our Abpromise guarantee covers the use of ab248495 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 63 kDa.
  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function

      Participates in the Wnt signaling pathway. Binds to DNA and acts as a repressor in the absence of CTNNB1, and as an activator in its presence. Necessary for the terminal differentiation of epidermal cells, the formation of keratohyalin granules and the development of the barrier function of the epidermis (By similarity). Down-regulates NQO1, leading to increased mitomycin c resistance.
    • Tissue specificity

      Detected in hair follicles and skin keratinocytes, and at lower levels in stomach epithelium.
    • Sequence similarities

      Belongs to the TCF/LEF family.
      Contains 1 HMG box DNA-binding domain.
    • Domain

      The putative Groucho interaction domain between the N-terminal CTNNB1 binding domain and the HMG-box is necessary for repression of the transactivation mediated by TCF7L1 and CTNNB1.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession Q9HCS4 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 83439 Human
      • Omim: 604652 Human
      • SwissProt: Q9HCS4 Human
      • Unigene: 516297 Human
      • Alternative names

        • bHLHb21 antibody
        • HMG box transcription factor 3 antibody
        • HMG box transcription factor antibody
        • OTTMUSP00000023419 antibody
        • T cell factor 3 antibody
        • TCF 3 antibody
        • TCF-3 antibody
        • TCF3 antibody
        • Tcf7l1 antibody
        • TF7L1_HUMAN antibody
        • Transcription factor 7 like 1 (T-cell specific, HMG-box) antibody
        • Transcription factor 7 like 1 antibody
        • Transcription factor 7-like 1 antibody
        see all

      Images

      • Western blot - Anti-TCF7L1 antibody [EPR2031] - BSA and Azide free (ab248495)
        Western blot - Anti-TCF7L1 antibody [EPR2031] - BSA and Azide free (ab248495)
        All lanes : Anti-TCF7L1 antibody [EPR2031] (ab133360) at 1/1000 dilution

        Lane 1 : A431 lysate
        Lane 2 : Human fetal small intestine lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 63 kDa



        This data was developed using ab133360, the same antibody clone in a different buffer formulation.

         

      • Anti-TCF7L1 antibody [EPR2031] - BSA and Azide free (ab248495)
        Anti-TCF7L1 antibody [EPR2031] - BSA and Azide free (ab248495)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet

      Certificate of Compliance

      To download a Certificate of Compliance, please enter your Lot number below:

    • References (0)

      Publishing research using ab248495? Please let us know so that we can cite the reference in this datasheet.

      ab248495 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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