Overview

  • Product name

  • Description

    Goat polyclonal to TCFL5
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide:

    C-KYIQERHGDSLKKE

    , corresponding to internal sequence amino acids 400-413 of Human TCFL5

  • Positive control

    • Human Testis lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab53546 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    ELISA: Peptide ELISA: Antibody detection limit dilution 1/64000.
    WB: Use at a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 48 kDa). An additional band of unknown identity was also consistently observed at 28kDa. This band was greatly reduced by incubation with the immunising peptide.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Putative transcription factor. Isoform 3 may play a role in early spermatogenesis.
    • Tissue specificity

      Isoform 3 is testis specific. Isoform 2 is pancreas specific.
    • Sequence similarities

      Contains 1 bHLH (basic helix-loop-helix) domain.
    • Developmental stage

      Isoform 3 is specifically expressed in primary spermatocytes at the pachytene stage, but not those at leptonema stage. Not expressed in other testicular cells, including spermatogonia located in the basal compartment of the seminiferous tubule or spermatids.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • bHLHe82 antibody
      • CHA antibody
      • Cha transcription factor antibody
      • E2BP-1 antibody
      • E2BP1 antibody
      • Figlb antibody
      • HPV-16 E2-binding protein 1 antibody
      • MGC46135 antibody
      • TCFL 5 antibody
      • TCFL5 antibody
      • TCFL5_HUMAN antibody
      • transcription factor-like 5 (basic helix-loop-helix) antibody
      • Transcription factor-like 5 protein antibody
      see all

    Images

    • All lanes : ab53546 antibody (0.3µg/ml) staining of TCFL5 in Human Testis lysate (35µg protein in RIPA buffer).

      Lane 1 : Without the immunising peptide
      Lane 2 : With the immunising peptide

      Predicted band size: 48 kDa
      Observed band size: 55 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 28 kDa. We are unsure as to the identity of these extra bands.



      Primary incubation was 1 hour. Detected by chemiluminescence.

    References

    ab53546 has not yet been referenced specifically in any publications.

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