Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, 5mg/ml Tris, pH 7.3
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab53546 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 48 kDa). An additional band of unknown identity was also consistently observed at 28kDa. This band was greatly reduced by incubation with the immunising peptide.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionPutative transcription factor. Isoform 3 may play a role in early spermatogenesis.
Tissue specificityIsoform 3 is testis specific. Isoform 2 is pancreas specific.
Sequence similaritiesContains 1 bHLH (basic helix-loop-helix) domain.
Developmental stageIsoform 3 is specifically expressed in primary spermatocytes at the pachytene stage, but not those at leptonema stage. Not expressed in other testicular cells, including spermatogonia located in the basal compartment of the seminiferous tubule or spermatids.
- Information by UniProt
- bHLHe82 antibody
- CHA antibody
- Cha transcription factor antibody
All lanes : ab53546 antibody (0.3µg/ml) staining of TCFL5 in Human Testis lysate (35µg protein in RIPA buffer).
Lane 1 : Without the immunising peptide
Lane 2 : With the immunising peptide
Predicted band size: 48 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Additional bands at: 28 kDa. We are unsure as to the identity of these extra bands.
Primary incubation was 1 hour. Detected by chemiluminescence.
ab53546 has not yet been referenced specifically in any publications.