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Epigenetics and Nuclear Signaling Transcription Cancer susceptibility Proto-oncogenes
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RecombinantRabMAb

Recombinant Anti-Tcl1 antibody [EPR3949] (ab108978)

  • Datasheet
  • SDS
Reviews (3)Q&A (1)References (1)

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Western blot - Anti-Tcl1 antibody [EPR3949] (ab108978)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tcl1 antibody [EPR3949] (ab108978)
  • Immunocytochemistry - Anti-Tcl1 antibody [EPR3949] (ab108978)
  • Western blot - Anti-Tcl1 antibody [EPR3949] (ab108978)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tcl1 antibody [EPR3949] (ab108978)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tcl1 antibody [EPR3949] (ab108978)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tcl1 antibody [EPR3949] (ab108978)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tcl1 antibody [EPR3949] (ab108978)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tcl1 antibody [EPR3949] (ab108978)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tcl1 antibody [EPR3949] (ab108978)
  • Flow Cytometry - Anti-Tcl1 antibody [EPR3949] (ab108978)
  • Flow Cytometry - Anti-Tcl1 antibody [EPR3949] (ab108978)
  • Anti-Tcl1 antibody [EPR3949] (ab108978)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3949] to Tcl1
  • Suitable for: WB, IHC-P, ICC, Flow Cyt
  • Reacts with: Human

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Secondary
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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
Protein
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Recombinant Human Tcl1 protein (ab87690)

View more associated products

Overview

  • Product name

    Anti-Tcl1 antibody [EPR3949]
    See all Tcl1 primary antibodies
  • Description

    Rabbit monoclonal [EPR3949] to Tcl1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Daudi and ramos cell lysates Human tonsil tissue Ramos whole cell lysates Jurkat (human acute T cell leukemia)
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR3949
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Proto-oncogenes
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Transcription factors

Associated products

  • Alternative Versions

    • Anti-Tcl1 antibody [EPR3949] - BSA and Azide free (ab199188)
    • PerCP Anti-Tcl1 antibody [EPR3949] (ab220513)
    • APC Anti-Tcl1 antibody [EPR3949] (ab221264)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Daudi whole cell lysate (ab3951)
    • Ramos whole cell lysate (ab3955)
  • Recombinant Protein

    • Recombinant Human Tcl1 protein (ab87690)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab108978 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 13 kDa.
IHC-P (2)
1/100 - 1/700. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Perform antigen retrieval

ICC
1/100 - 1/250.
Flow Cyt
1/400.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Unpurified format: 1/100 - 1/1000.

 

Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 13 kDa.
IHC-P
1/100 - 1/700. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Perform antigen retrieval

ICC
1/100 - 1/250.
Flow Cyt
1/400.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Unpurified format: 1/100 - 1/1000.

 

Target

  • Function

    Enhances the phosphorylation and activation of AKT1, AKT2 and AKT3. Promotes nuclear translocation of AKT1. Enhances cell proliferation, stabilizes mitochondrial membrane potential and promotes cell survival.
  • Tissue specificity

    Restricted in the T-cell lineage to immature thymocytes and activated peripheral lymphocytes. Preferentially expressed early in T- and B-lymphocyte differentiation.
  • Involvement in disease

    Note=Chromosomal aberrations activating TCL1A are found in chronic T-cell leukemias (T-CLL). Translocation t(14;14)(q11;q32); translocation t(7;14)(q35;q32); inversion inv(14)(q11;q32) that involves the T-cell receptor alpha/delta loci.
  • Sequence similarities

    Belongs to the TCL1 family.
  • Cellular localization

    Cytoplasm. Nucleus. Microsome. Endoplasmic reticulum. Microsomal fraction.
  • Target information above from: UniProt accession P56279 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 8115 Human
    • Omim: 186960 Human
    • SwissProt: P56279 Human
    • Unigene: 2484 Human
    • Alternative names

      • Anti TCL1A antibody
      • Lymphoma/leukemia, T-cell antibody
      • Oncogene TCL-1 antibody
      • Oncogene TCL1 antibody
      • P14 TCL1 antibody
      • P14 TCL1 protein antibody
      • Protein p14 TCL1 antibody
      • T cell leukemia 1 antibody
      • T cell lymphoma 1 antibody
      • T cell lymphoma 1A antibody
      • T-cell leukemia/lymphoma 1A antibody
      • T-cell leukemia/lymphoma protein 1A antibody
      • TCL 1 protein antibody
      • TCL1 antibody
      • TCL1 oncogene antibody
      • TCL1 PEN antibody
      • Tcl1a antibody
      • TCL1A antibody antibody
      • TCL1A_HUMAN antibody
      see all

    Images

    • Western blot - Anti-Tcl1 antibody [EPR3949] (ab108978)
      Western blot - Anti-Tcl1 antibody [EPR3949] (ab108978)
      All lanes : Anti-Tcl1 antibody [EPR3949] (ab108978) at 1/1000 dilution

      Lane 1 : Ramos (human Burkitt's lymphoma) whole cell lysate
      Lane 2 : Daudi (human Burkitt's lymphoma) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

      Predicted band size: 13 kDa
      Additional bands at: 13 kDa. We are unsure as to the identity of these extra bands.



      Blocking buffer: 5% NFDM/TBST

      Diluting buffer: 5% NFDM/TBST

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tcl1 antibody [EPR3949] (ab108978)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tcl1 antibody [EPR3949] (ab108978)

      ab108978 staining Tcl1 in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/700. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at 1/500.

      Negative control 1: PBS in place of primary antibody.

    • Immunocytochemistry - Anti-Tcl1 antibody [EPR3949] (ab108978)
      Immunocytochemistry - Anti-Tcl1 antibody [EPR3949] (ab108978)

      ab108978 staining Tcl1 in Jurkat (human acute T cell leukemia) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/200. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at 1/1000. ab7291 and ab150120 were used as counterstains for primary antibody ab108978 and secondary antibody ab150077 respectively and DAPI was used as a nuclear counterstain.

      Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (ab150120)
      Negative control 2: Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077)

    • Western blot - Anti-Tcl1 antibody [EPR3949] (ab108978)
      Western blot - Anti-Tcl1 antibody [EPR3949] (ab108978)
      All lanes : Anti-Tcl1 antibody [EPR3949] (ab108978) at 1/1000 dilution

      Lane 1 : Daudi cell lysate
      Lane 2 : Ramos cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Standard HRP labelled goat anti-goat. at 1/2000 dilution

      Predicted band size: 13 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tcl1 antibody [EPR3949] (ab108978)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tcl1 antibody [EPR3949] (ab108978)

      Immunohistochemical analysis of paraffin-embedded Human tonsil tissue using ab108978.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tcl1 antibody [EPR3949] (ab108978)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tcl1 antibody [EPR3949] (ab108978)

      ab108978 showing negative staining in Skeletal muscle tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tcl1 antibody [EPR3949] (ab108978)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tcl1 antibody [EPR3949] (ab108978)

      ab108978 showing positive staining in Normal spleen tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tcl1 antibody [EPR3949] (ab108978)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tcl1 antibody [EPR3949] (ab108978)

      ab108978 showing negative staining in Normal brain tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tcl1 antibody [EPR3949] (ab108978)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tcl1 antibody [EPR3949] (ab108978)

      ab108978 showing negative staining in Normal kidney tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tcl1 antibody [EPR3949] (ab108978)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tcl1 antibody [EPR3949] (ab108978)

      ab108978 showing negative staining in Normal stomach tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Flow Cytometry - Anti-Tcl1 antibody [EPR3949] (ab108978)
      Flow Cytometry - Anti-Tcl1 antibody [EPR3949] (ab108978)

      ab108978 staining Tcl1 in the human cell line Ramos (human Burkitt's lymphoma) by flow cytometry. Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/400. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

      Isoytype control: Rabbit monoclonal IgG (Black)

      Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

    • Flow Cytometry - Anti-Tcl1 antibody [EPR3949] (ab108978)
      Flow Cytometry - Anti-Tcl1 antibody [EPR3949] (ab108978)
      Overlay histogram showing Ramos cells stained with ab108978 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108978, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Ramos cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
    • Anti-Tcl1 antibody [EPR3949] (ab108978)
      Anti-Tcl1 antibody [EPR3949] (ab108978)

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab108978? Please let us know so that we can cite the reference in this datasheet.

    ab108978 has been referenced in 1 publication.

    • Punt S  et al. Whole-transcriptome analysis of flow-sorted cervical cancer samples reveals that B cell expressed TCL1A is correlated with improved survival. Oncotarget 6:38681-94 (2015). PubMed: 26299617

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-4 of 4 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Tcl1 antibody [EPR3949]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Baboon Tissue sections (Prepubertal Testis)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Sodium Citrate
    Permeabilization
    No
    Specification
    Prepubertal Testis
    Blocking step
    5% Serum + 3% BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 28 2017

    Immunohistochemistry (Frozen sections) abreview for Anti-Tcl1 antibody [EPR3949]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 30% · Temperature: 24°C
    Sample
    Human Tissue sections (Lymph Node)
    Specification
    Lymph Node
    Permeabilization
    Yes - TritonX
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 26 2013

    Question

    What are the positive controls and staining procedures for ab108978 and ab91211?

    Read More

    Abcam community

    Verified customer

    Asked on Dec 14 2011

    Answer

    Thank you for contacting us regarding ab108978 and ab91211. I've attached the recommended Abcam IHC-P protocol and a troubleshooting guide for you. Here is a link to the protocol as well: https://www.abcam.com/index.html?pageconfig=resource&rid=11384 Ab108978: We recommend using at a dilution of 1/100 - 1/250. Perform antigen retrieval is recommended as well. Positive controls for this antibody are Daudi and ramos cell lysates Human tonsil tissue Ab91211: Use at assay dependent concentrations, we usually recommend about 5ug/ml. Positive controls for this antibody are  Daudi cells.

    Read More

    Abcam Scientific Support

    Answered on Dec 14 2011

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Tcl1 antibody [EPR3949]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (tonsil)
    Specification
    tonsil
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: EDTA pH9.0, 20 min, 100C
    Permeabilization
    No
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 12 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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