Overview

  • Product name

    Anti-Tcl1 antibody [EPR3949] (Allophycocyanin)
    See all Tcl1 primary antibodies
  • Description

    Rabbit monoclonal [EPR3949] to Tcl1 (Allophycocyanin)
  • Host species

    Rabbit
  • Conjugation

    Allophycocyanin. Ex: 645nm, Em: 660nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Tcl1 aa 1-100. The exact sequence is proprietary.
    Database link: P56279

  • Positive control

    • Flow Cyt: HeLa cells
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab221264 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50000.

The cellular localisation of this product has been verified in ICC/IF.

Target

  • Function

    Enhances the phosphorylation and activation of AKT1, AKT2 and AKT3. Promotes nuclear translocation of AKT1. Enhances cell proliferation, stabilizes mitochondrial membrane potential and promotes cell survival.
  • Tissue specificity

    Restricted in the T-cell lineage to immature thymocytes and activated peripheral lymphocytes. Preferentially expressed early in T- and B-lymphocyte differentiation.
  • Involvement in disease

    Note=Chromosomal aberrations activating TCL1A are found in chronic T-cell leukemias (T-CLL). Translocation t(14;14)(q11;q32); translocation t(7;14)(q35;q32); inversion inv(14)(q11;q32) that involves the T-cell receptor alpha/delta loci.
  • Sequence similarities

    Belongs to the TCL1 family.
  • Cellular localization

    Cytoplasm. Nucleus. Microsome. Endoplasmic reticulum. Microsomal fraction.
  • Information by UniProt
  • Database links

  • Alternative names

    • Anti TCL1A antibody
    • Lymphoma/leukemia, T-cell antibody
    • Oncogene TCL-1 antibody
    • Oncogene TCL1 antibody
    • P14 TCL1 antibody
    • P14 TCL1 protein antibody
    • Protein p14 TCL1 antibody
    • T cell leukemia 1 antibody
    • T cell lymphoma 1 antibody
    • T cell lymphoma 1A antibody
    • T-cell leukemia/lymphoma 1A antibody
    • T-cell leukemia/lymphoma protein 1A antibody
    • TCL 1 protein antibody
    • TCL1 antibody
    • TCL1 oncogene antibody
    • TCL1 PEN antibody
    • Tcl1a antibody
    • TCL1A antibody antibody
    • TCL1A_HUMAN antibody
    see all

Images

  • Overlay histogram showing HeLa cells stained with ab221264 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab221264, 1/50000 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was Rabbit IgG (monoclonal) Allophycocyanin used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter.

    This antibody gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

References

ab221264 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab221264.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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