Overview

  • Product name

    Anti-Tcl1 antibody [EPR3949] - BSA and Azide free
    See all Tcl1 primary antibodies
  • Description

    Rabbit monoclonal [EPR3949] to Tcl1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC, WB, ICC/IF, IHC-Fr, Flow Cyt, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Tcl1 aa 1-100. The exact sequence is proprietary.

  • General notes

    ab199188 is the carrier-free version of ab108978 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab199188 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab199188 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 13 kDa.
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Perform antigen retrieval

 

Target

  • Function

    Enhances the phosphorylation and activation of AKT1, AKT2 and AKT3. Promotes nuclear translocation of AKT1. Enhances cell proliferation, stabilizes mitochondrial membrane potential and promotes cell survival.
  • Tissue specificity

    Restricted in the T-cell lineage to immature thymocytes and activated peripheral lymphocytes. Preferentially expressed early in T- and B-lymphocyte differentiation.
  • Involvement in disease

    Note=Chromosomal aberrations activating TCL1A are found in chronic T-cell leukemias (T-CLL). Translocation t(14;14)(q11;q32); translocation t(7;14)(q35;q32); inversion inv(14)(q11;q32) that involves the T-cell receptor alpha/delta loci.
  • Sequence similarities

    Belongs to the TCL1 family.
  • Cellular localization

    Cytoplasm. Nucleus. Microsome. Endoplasmic reticulum. Microsomal fraction.
  • Information by UniProt
  • Database links

  • Alternative names

    • Anti TCL1A antibody
    • Lymphoma/leukemia, T-cell antibody
    • Oncogene TCL-1 antibody
    • Oncogene TCL1 antibody
    • P14 TCL1 antibody
    • P14 TCL1 protein antibody
    • Protein p14 TCL1 antibody
    • T cell leukemia 1 antibody
    • T cell lymphoma 1 antibody
    • T cell lymphoma 1A antibody
    • T-cell leukemia/lymphoma 1A antibody
    • T-cell leukemia/lymphoma protein 1A antibody
    • TCL 1 protein antibody
    • TCL1 antibody
    • TCL1 oncogene antibody
    • TCL1 PEN antibody
    • Tcl1a antibody
    • TCL1A antibody antibody
    • TCL1A_HUMAN antibody
    see all

Images

  • ab108978 staining Tcl1 in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/700. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at 1/500.

    Negative control 1: PBS in place of primary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108978).

  • ab108978 staining Tcl1 in the human cell line Ramos (human Burkitt's lymphoma) by flow cytometry. Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/400. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

    Isoytype control: Rabbit monoclonal IgG (Black)

    Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108978).

  • ab108978 staining Tcl1 in Jurkat (human acute T cell leukemia) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/200. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at 1/1000. ab7291 and ab150120 were used as counterstains for primary antibody ab108978 and secondary antibody ab150077 respectively and DAPI was used as a nuclear counterstain.

    Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (ab150120)
    Negative control 2: Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108978).

  • Overlay histogram showing Ramos cells stained with ab108978 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108978, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Ramos cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108978).

  • Immunohistochemical analysis of paraffin-embedded Human tonsil tissue using ab108978.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108978).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • ab108978 showing negative staining in Skeletal muscle tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108978).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • ab108978 showing positive staining in Normal spleen tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108978).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • ab108978 showing negative staining in Normal brain tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108978).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • ab108978 showing negative staining in Normal kidney tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108978).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • ab108978 showing negative staining in Normal stomach tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108978).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

References

ab199188 has not yet been referenced specifically in any publications.

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