Overview

  • Product name
    Anti-Tcl1 antibody [SP248]
    See all Tcl1 primary antibodies
  • Description
    Rabbit monoclonal [SP248] to Tcl1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Tcl1 aa 50 to the C-terminus (internal sequence). The exact sequence is proprietary.
    Database link: P56279

  • Positive control
    • WB: Ramos cell lysate. IHC-P: Human tonsil tissue. Flow Cytometry: Ramos cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab225718 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/400. Predicted molecular weight: 13 kDa.

Primary incubation for 1 hour at room temperature.

IHC-P 1/100.

Boil tissue section in citrate buffer pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.

Primary incubation for 10 minutes at room temperature.

Flow Cyt 1/400.

Primary incubation for 30 minutes at 4°C.

Target

  • Function
    Enhances the phosphorylation and activation of AKT1, AKT2 and AKT3. Promotes nuclear translocation of AKT1. Enhances cell proliferation, stabilizes mitochondrial membrane potential and promotes cell survival.
  • Tissue specificity
    Restricted in the T-cell lineage to immature thymocytes and activated peripheral lymphocytes. Preferentially expressed early in T- and B-lymphocyte differentiation.
  • Involvement in disease
    Note=Chromosomal aberrations activating TCL1A are found in chronic T-cell leukemias (T-CLL). Translocation t(14;14)(q11;q32); translocation t(7;14)(q35;q32); inversion inv(14)(q11;q32) that involves the T-cell receptor alpha/delta loci.
  • Sequence similarities
    Belongs to the TCL1 family.
  • Cellular localization
    Cytoplasm. Nucleus. Microsome. Endoplasmic reticulum. Microsomal fraction.
  • Information by UniProt
  • Database links
  • Alternative names
    • Anti TCL1A antibody
    • Lymphoma/leukemia, T-cell antibody
    • Oncogene TCL-1 antibody
    • Oncogene TCL1 antibody
    • P14 TCL1 antibody
    • P14 TCL1 protein antibody
    • Protein p14 TCL1 antibody
    • T cell leukemia 1 antibody
    • T cell lymphoma 1 antibody
    • T cell lymphoma 1A antibody
    • T-cell leukemia/lymphoma 1A antibody
    • T-cell leukemia/lymphoma protein 1A antibody
    • TCL 1 protein antibody
    • TCL1 antibody
    • TCL1 oncogene antibody
    • TCL1 PEN antibody
    • Tcl1a antibody
    • TCL1A antibody antibody
    • TCL1A_HUMAN antibody
    see all

Images

  • Anti-Tcl1 antibody [SP248] (ab225718) at 1/400 dilution + Ramos (human Burkitt's lymphoma cell line) cell lysate

    Predicted band size: 13 kDa

  • Formalin-fixed, paraffin-embedded human tonsil tissue stained for Tcl1 using ab225718 at 1/100 dilution in immunohistochemical analysis.

  • Flow cytometric analysis of Ramos (human Burkitt's lymphoma cell line) cells treated with ab225718 at 1/400 dilution (green)  compared with a negative control of rabbit IgG (blue).

References

ab225718 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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