Overview

  • Product name

  • Description

    Rabbit polyclonal to TCOF1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ChIP, WB, IHC-P, ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human TCOF1.

  • Positive control

    • Human lung carcinoma tissue and extracts from Jurkat cells
  • General notes

     This product was previously labelled as Treacher Collins syndrome protein

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride

    Without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab65212 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration. PubMed: 20591827
WB 1/500 - 1/1000. Detects a band of approximately 152 kDa (predicted molecular weight: 152 kDa).
IHC-P 1/50 - 1/100.
ELISA 1/10000.

Target

  • Function

    May be involved in nucleolar-cytoplasmic transport. May play a fundamental role in early embryonic development, particularly in development of the craniofacial complex.
  • Involvement in disease

    Defects in TCOF1 are the cause of Treacher Collins syndrome type 1 (TCS1) [MIM:154500]. It is a form of Treacher Collins syndrome, a disorder of craniofacial development. Treacher Collins syndrome is characterized by a combination of bilateral downward slanting of the palpebral fissures, colobomas of the lower eyelids with a paucity of eyelashes medial to the defect, hypoplasia of the facial bones, cleft palate, malformation of the external ears, atresia of the external auditory canals, and bilateral conductive hearing loss.
  • Sequence similarities

    Contains 1 LisH domain.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus > nucleolus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Mandibulofacial dysostosis antibody
    • MFD1 antibody
    • Nucleolar trafficking phosphoprotein antibody
    • TCOF 1 antibody
    • TCOF_HUMAN antibody
    • TCOF1 antibody
    • TCS antibody
    • TCS1 antibody
    • Treacher Collins Franceschetti syndrome 1 antibody
    • Treacher Collins syndrome antibody
    • Treacher Collins syndrome protein antibody
    • Treacle antibody
    • Treacle protein antibody
    see all

Images

  • All lanes : Anti-TCOF1 antibody (ab65212) at 1/500 dilution

    Lane 1 : Extracts from Jurkat cells
    Lane 2 : Extracts from Jurkat cells, plus immunising peptide

    Predicted band size: 152 kDa
    Observed band size: 152 kDa



    The amount of positive control loading for the WB is 5-30 ug of total protein.
    The amount of the peptide for the WB is 5-10 ug.
  • Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using ab65212 at a 1:50 dilution.
    Left image untreated.
    Right image treated with immunising peptide.

References

This product has been referenced in:

  • Franek M  et al. Nucleolar Reorganization Upon Site-Specific Double-Strand Break Induction. J Histochem Cytochem 64:669-686 (2016). Read more (PubMed: 27680669) »
  • Stixová L  et al. HP1ß-dependent recruitment of UBF1 to irradiated chromatin occurs simultaneously with CPDs. Epigenetics Chromatin 7:39 (2014). ICC/IF ; Mouse . Read more (PubMed: 25587355) »
See all 3 Publications for this product

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