Key features and details
- Rabbit polyclonal to TCOF1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TCOF1 antibody
See all TCOF1 primary antibodies
DescriptionRabbit polyclonal to TCOF1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human TCOF1.
This product was previously labelled as Treacher Collins syndrome protein
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab65212 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 152 kDa (predicted molecular weight: 152 kDa).|
|IHC-P||1/50 - 1/100.|
FunctionMay be involved in nucleolar-cytoplasmic transport. May play a fundamental role in early embryonic development, particularly in development of the craniofacial complex.
Involvement in diseaseDefects in TCOF1 are the cause of Treacher Collins syndrome type 1 (TCS1) [MIM:154500]. It is a form of Treacher Collins syndrome, a disorder of craniofacial development. Treacher Collins syndrome is characterized by a combination of bilateral downward slanting of the palpebral fissures, colobomas of the lower eyelids with a paucity of eyelashes medial to the defect, hypoplasia of the facial bones, cleft palate, malformation of the external ears, atresia of the external auditory canals, and bilateral conductive hearing loss.
Sequence similaritiesContains 1 LisH domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus > nucleolus.
- Information by UniProt
- Mandibulofacial dysostosis antibody
- MFD1 antibody
- Nucleolar trafficking phosphoprotein antibody
All lanes : Anti-TCOF1 antibody (ab65212) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells
Lane 2 : Extracts from Jurkat cells, plus immunising peptide
Predicted band size: 152 kDa
Observed band size: 152 kDa
The amount of positive control loading for the WB is 5-30 ug of total protein.The amount of the peptide for the WB is 5-10 ug.
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using ab65212 at a 1:50 dilution.
Left image untreated.Right image treated with immunising peptide.
ab65212 has been referenced in 3 publications.
- Franek M et al. Nucleolar Reorganization Upon Site-Specific Double-Strand Break Induction. J Histochem Cytochem 64:669-686 (2016). PubMed: 27680669
- Stixová L et al. HP1ß-dependent recruitment of UBF1 to irradiated chromatin occurs simultaneously with CPDs. Epigenetics Chromatin 7:39 (2014). ICC/IF ; Mouse . PubMed: 25587355
- Zentner GE et al. CHD7 functions in the nucleolus as a positive regulator of ribosomal RNA biogenesis. Hum Mol Genet 19:3491-501 (2010). WB, ChIP ; Mouse . PubMed: 20591827