Overview

  • Product name
  • Description
    Goat polyclonal to TCP1 alpha
  • Host species
    Goat
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Macaque monkey, Gorilla
  • Immunogen

    Synthetic peptide: SYEDAVHSGALND, corresponding to C terminal amino acids 544-556 of Human TCP1 alpha.

  • Positive control
    • Human lung and testis lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab10180 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
WB
  • Application notes
    Peptide ELISA: antibody detection limit dilution 1/32000.
    WB: Use at a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa according to NP_110379).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.
    • Sequence similarities
      Belongs to the TCP-1 chaperonin family.
    • Cellular localization
      Cytoplasm. Cytoplasm > cytoskeleton > centrosome.
    • Information by UniProt
    • Database links
    • Alternative names
      • AI528772 antibody
      • c-cpn antibody
      • CCT alpha antibody
      • CCT antibody
      • CCT-alpha antibody
      • CCT1 antibody
      • Ccta antibody
      • CCTalpha antibody
      • D6S230E antibody
      • MGC133746 antibody
      • p63 antibody
      • T complex 1 antibody
      • T complex protein 1 alpha subunit antibody
      • T complex protein 1 antibody
      • T-complex homolog TCP1 antibody
      • T-complex protein 1 subunit alpha antibody
      • T-complex protein 1 subunit alpha B antibody
      • Tailless complex polypeptide 1 antibody
      • Tailless complex polypeptide 1A antibody
      • Tailless complex polypeptide 1B antibody
      • TCP 1 alpha antibody
      • Tcp-1 antibody
      • TCP-1-alpha antibody
      • TCP1 antibody
      • TCPA_HUMAN antibody
      • Tp63 antibody
      • TRic antibody
      see all

    Images

    • ab10180 staining (1µg/ml) of human lung lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by Western blot using chemiluminescence. ab10180 staining (1µg/ml) of human lung lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by Western blot using chemiluminescence.
    • ab10180 (10µg/ml) staining paraffin embedded Human Testis, microwaved antigen retreival with Tris/EDTA buffer pH9, HRP-staining.

    References

    This product has been referenced in:
    • Liu SL  et al. Orthogonal lipid sensors identify transbilayer asymmetry of plasma membrane cholesterol. Nat Chem Biol 13:268-274 (2017). Read more (PubMed: 28024150) »
    • Ryan T  et al. Identification of Novel Ryanodine Receptor 1 (RyR1) Protein Interaction with Calcium Homeostasis Endoplasmic Reticulum Protein (CHERP). J Biol Chem 286:17060-8 (2011). WB ; Human . Read more (PubMed: 21454501) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    Answer

    Thank you for your email. So far, ab10180 has only been tested for cross-reactivity with Human. It is expected to cross-react with Chimpanzee (100% identity with immunogen), Gorilla (100% identity with immunogen) and Macaque Monkey (100% identity with immunogen) due to sequence homology. It has not yet been tested in other species. The peptide sequence is listed on the online datasheet, so you may want to BLAST the sequence to see what the homology is with mouse. Please let me know if you require any further assistance.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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