Key features and details
- Rat monoclonal [91a] to TCP1 alpha/CCTA
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TCP1 alpha/CCTA antibody [91a]
See all TCP1 alpha/CCTA primary antibodies
DescriptionRat monoclonal [91a] to TCP1 alpha/CCTA
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Mouse TCP1 alpha/CCTA (C terminal).
Database link: P11983
- WB: A431 and HEK-293 cell lysates. ICC/IF: HeLa cells.
This product was previously labelled as TCP1 alpha
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityProtein G purified
Purification notesPurified from TCS.
Our Abpromise guarantee covers the use of ab240903 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 60 kDa.|
FunctionMolecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.
Sequence similaritiesBelongs to the TCP-1 chaperonin family.
Cellular localizationCytoplasm. Cytoplasm > cytoskeleton > centrosome.
- Information by UniProt
- AI528772 antibody
- c-cpn antibody
- CCT alpha antibody
All lanes : Anti-TCP1 alpha/CCTA antibody [91a] (ab240903) at 1/1000 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line) cell lysate
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Predicted band size: 60 kDa
Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa (heat shocked for 1 hour at 42°)(human epithelial cell line from cervix adenocarcinoma) cells labeling TCP1 alpha/CCTA with ab240903 at 1/100 dilution for 12 hours at 4°C, followed by R-PE Goat Anti-Rat (yellow) at 1/200 dilution for 2 hours at room temperature (yellow). The nuclear counter stain is DAPI (blue).
A: DAPI, B: TCP1 alpha/CCTA, C: Composite.
Immunofluorescent analysis of 2% paraformaldehyde-fixed HeLa (heat shocked for 1 hour at 42°) (human epithelial cell line from cervix adenocarcinoma) cells labeling TCP1 alpha/CCTA with ab240903 at 1/100 dilution for 12 hours at 4°C, followed by APC Goat Anti-Rat at 1/200 dilution for 2 hours at room temperature (red). The nuclear counter stain is DAPI (blue).
A: DAPI, B: TCP1 alpha/CCTA, C: Composite.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab240903 has not yet been referenced specifically in any publications.