Anti-TCP1 alpha/CCTA antibody [91a] (ab240903)
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Overview
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Product name
Anti-TCP1 alpha/CCTA antibody [91a]
See all TCP1 alpha/CCTA primary antibodies -
Description
Rat monoclonal [91a] to TCP1 alpha/CCTA -
Host species
Rat -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Mouse TCP1 alpha/CCTA (C terminal).
Database link: P11983 -
Positive control
- WB: A431 and HEK-293 cell lysates. ICC/IF: HeLa cells.
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General notes
This product was previously labelled as TCP1 alpha
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
91a -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab240903 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Predicted molecular weight: 60 kDa. | |
| ICC/IF | 1/100. |
Target
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Function
Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin. -
Sequence similarities
Belongs to the TCP-1 chaperonin family. -
Cellular localization
Cytoplasm. Cytoplasm > cytoskeleton > centrosome. - Information by UniProt
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Database links
- Entrez Gene: 6950 Human
- Omim: 186980 Human
- SwissProt: P17987 Human
- Unigene: 363137 Human
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Alternative names
- AI528772 antibody
- c-cpn antibody
- CCT alpha antibody
see all
Images
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![Western blot - Anti-TCP1 alpha/CCTA antibody [91a] (ab240903)](https://www.abcam.com/ps/products/240/ab240903/Images/ab240903-319754-anti-tcp1-alpha-antibody-91a-western-blot.jpg)
Western blot - Anti-TCP1 alpha/CCTA antibody [91a] (ab240903)All lanes : Anti-TCP1 alpha/CCTA antibody [91a] (ab240903) at 1/1000 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line) cell lysate
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Predicted band size: 60 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-TCP1 alpha/CCTA antibody [91a] (ab240903)](https://www.abcam.com/ps/products/240/ab240903/Images/ab240903-319756-anti-tcp1-alpha-antibody-91a-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-TCP1 alpha/CCTA antibody [91a] (ab240903)Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa (heat shocked for 1 hour at 42°)(human epithelial cell line from cervix adenocarcinoma) cells labeling TCP1 alpha/CCTA with ab240903 at 1/100 dilution for 12 hours at 4°C, followed by R-PE Goat Anti-Rat (yellow) at 1/200 dilution for 2 hours at room temperature (yellow). The nuclear counter stain is DAPI (blue).
A: DAPI, B: TCP1 alpha/CCTA, C: Composite.
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![Immunocytochemistry/ Immunofluorescence - Anti-TCP1 alpha/CCTA antibody [91a] (ab240903)](https://www.abcam.com/ps/products/240/ab240903/Images/ab240903-319755-anti-tcp1-alpha-antibody-91a-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-TCP1 alpha/CCTA antibody [91a] (ab240903)Immunofluorescent analysis of 2% paraformaldehyde-fixed HeLa (heat shocked for 1 hour at 42°) (human epithelial cell line from cervix adenocarcinoma) cells labeling TCP1 alpha/CCTA with ab240903 at 1/100 dilution for 12 hours at 4°C, followed by APC Goat Anti-Rat at 1/200 dilution for 2 hours at room temperature (red). The nuclear counter stain is DAPI (blue).
A: DAPI, B: TCP1 alpha/CCTA, C: Composite.
Datasheets and documents
References
ab240903 has not yet been referenced specifically in any publications.
