Key features and details
- Rabbit polyclonal to TCP1 alpha/CCTA
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TCP1 alpha/CCTA antibody
See all TCP1 alpha/CCTA primary antibodies
DescriptionRabbit polyclonal to TCP1 alpha/CCTA
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide within Human TCP1 alpha/CCTA aa 506-556. The exact sequence is proprietary. (NP_110379.2).
Database link: P17987
- IHC-P: Human prostate carcinoma and breast carcinoma tissues. WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HeLa whole cell lysate.
This product was previously labelled as TCP1 alpha
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab225695 was affinity purified using an epitope specific to TCP1 alpha/CCTA immobilized on solid support.
Our Abpromise guarantee covers the use of ab225695 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 60 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
|IHC-P||1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionMolecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.
Sequence similaritiesBelongs to the TCP-1 chaperonin family.
Cellular localizationCytoplasm. Cytoplasm > cytoskeleton > centrosome.
- Information by UniProt
- AI528772 antibody
- c-cpn antibody
- CCT alpha antibody
All lanes : Anti-TCP1 alpha/CCTA antibody (ab225695) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 60 kDa
Exposure time: 3 seconds
Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for TCP1 alpha/CCTA using ab225695 at 1/200 dilution in immunohistochemical analysis. Detection: DAB staining.
Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for TCP1 alpha/CCTA using ab225695 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB staining.
TCP1 alpha/CCTA was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225695 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab225695 at 0.4 µg/ml.
Lane 1: ab225695 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab225695 has not yet been referenced specifically in any publications.