Product nameAnti-TCP1 alpha/CCTA antibody [EPR4081]
See all TCP1 alpha/CCTA primary antibodies
DescriptionRabbit monoclonal [EPR4081] to TCP1 alpha/CCTA
Tested applicationsSuitable for: ICC/IF, WB, IHC-P, IPmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Human
corresponding to Human TCP1 alpha/CCTA (N terminal).
Database link: P17987
- HeLa, ECV-304, A459 and 293T cell lysates, Human colon tissue, Human kidney tissue and HeLa cells
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as TCP1 alpha
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab92587 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. The use of an HRP/AP polymerized antibody is recommended for a secondary antibody.
IP: 1/10 - 1/100.
WB: 1/10000 - 1/50000. Predicted molecular weight: 60 kDa.
Is unsuitable for Flow Cyt.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionMolecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.
Sequence similaritiesBelongs to the TCP-1 chaperonin family.
Cellular localizationCytoplasm. Cytoplasm > cytoskeleton > centrosome.
- Information by UniProt
- AI528772 antibody
- c-cpn antibody
- CCT alpha antibody
All lanes : Anti-TCP1 alpha/CCTA antibody [EPR4081] (ab92587) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : ECV-304 cell lysate
Lane 3 : A549 cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 60 kDa
ab92587 at 1/100 dilution staining TCP1 alpha/CCTA in (1) Human colon tissue and (2) Human kidney tissue by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ab92587 at 1/250 dilution staining TCP1 alpha/CCTA in HeLa cells by Immunofluorescence.
ab92587 has not yet been referenced specifically in any publications.