Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4081] to TCP1 alpha/CCTA
- Suitable for: ICC/IF, WB, IHC-P, IP
- Reacts with: Human
Product nameAnti-TCP1 alpha/CCTA antibody [EPR4081]
See all TCP1 alpha/CCTA primary antibodies
DescriptionRabbit monoclonal [EPR4081] to TCP1 alpha/CCTA
Tested applicationsSuitable for: ICC/IF, WB, IHC-P, IPmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Human
corresponding to Human TCP1 alpha/CCTA (N terminal).
Database link: P17987
- HeLa, ECV-304, A459 and 293T cell lysates, Human colon tissue, Human kidney tissue and HeLa cells
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as TCP1 alpha
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab92587 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. The use of an HRP/AP polymerized antibody is recommended for a secondary antibody.
IP: 1/10 - 1/100.
WB: 1/10000 - 1/50000. Predicted molecular weight: 60 kDa.
Is unsuitable for Flow Cyt.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionMolecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.
Sequence similaritiesBelongs to the TCP-1 chaperonin family.
Cellular localizationCytoplasm. Cytoplasm > cytoskeleton > centrosome.
- Information by UniProt
- AI528772 antibody
- c-cpn antibody
- CCT alpha antibody
All lanes : Anti-TCP1 alpha/CCTA antibody [EPR4081] (ab92587) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : ECV-304 cell lysate
Lane 3 : A549 cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 60 kDa
ab92587 at 1/100 dilution staining TCP1 alpha/CCTA in (1) Human colon tissue and (2) Human kidney tissue by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ab92587 at 1/250 dilution staining TCP1 alpha/CCTA in HeLa cells by Immunofluorescence.
ab92587 has not yet been referenced specifically in any publications.