Recombinant
RabMAb

Recombinant Anti-TCP1 alpha/CCTA antibody [EPR4082] - BSA and Azide free (ab239946)

Overview

  • Product name

    Anti-TCP1 alpha/CCTA antibody [EPR4082] - BSA and Azide free
    See all TCP1 alpha/CCTA primary antibodies
  • Description

    Rabbit monoclonal [EPR4082] to TCP1 alpha/CCTA - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, ICC/IF, IHC-P, WBmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Mouse, Rat, Rabbit, Human
  • Immunogen

    Synthetic peptide within Human TCP1 alpha/CCTA aa 500-600. The exact sequence is proprietary.

  • General notes

    ab239946 is the carrier-free version of ab109126 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab239946 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as TCP1 alpha

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab239946 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 60 kDa.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function

      Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.
    • Sequence similarities

      Belongs to the TCP-1 chaperonin family.
    • Cellular localization

      Cytoplasm. Cytoplasm > cytoskeleton > centrosome.
    • Information by UniProt
    • Database links

    • Alternative names

      • AI528772 antibody
      • c-cpn antibody
      • CCT alpha antibody
      • CCT antibody
      • CCT-alpha antibody
      • CCT1 antibody
      • Ccta antibody
      • CCTalpha antibody
      • D6S230E antibody
      • MGC133746 antibody
      • p63 antibody
      • T complex 1 antibody
      • T complex protein 1 alpha subunit antibody
      • T complex protein 1 antibody
      • T-complex homolog TCP1 antibody
      • T-complex protein 1 subunit alpha antibody
      • T-complex protein 1 subunit alpha B antibody
      • Tailless complex polypeptide 1 antibody
      • Tailless complex polypeptide 1A antibody
      • Tailless complex polypeptide 1B antibody
      • TCP 1 alpha antibody
      • Tcp-1 antibody
      • TCP-1-alpha antibody
      • TCP1 antibody
      • TCPA_HUMAN antibody
      • Tp63 antibody
      • TRic antibody
      see all

    Images

    • ab109126 at 1:50 dilution (5μg in 1mg lysate) immunoprecipitating TCP1 alpha/CCTA in ECV-304 (human urinary bladder cancer epithelial cell) whole cell lysate. 

       

      Lane 1: ECV-304 (human urinary bladder cancer epithelial cell) whole cell lysate

      Lane 2: PBS instead of ab109126 in ECV-304 whole cell lysate


      For western blotting, Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG was used as the secondary antibody at 1:1500 dilution.

      Blocking and diluting buffer: 5% NFDM/TBST.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109126).

    • ab109126, at 1/100 dilution staining TCP1 in paraffin-embedded Human testis tissue, by Immunohistochemistry.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109126).

    References

    ab239946 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab239946.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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