Product nameAnti-TCP1 alpha/CCTA antibody [EPR4082] - BSA and Azide free
See all TCP1 alpha/CCTA primary antibodies
DescriptionRabbit monoclonal [EPR4082] to TCP1 alpha/CCTA - BSA and Azide free
Tested applicationsSuitable for: IP, ICC/IF, IHC-P, WBmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Mouse, Rat, Rabbit, Human
Synthetic peptide within Human TCP1 alpha/CCTA aa 500-600. The exact sequence is proprietary.
ab239946 is the carrier-free version of ab109126 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab239946 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as TCP1 alpha
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
Our Abpromise guarantee covers the use of ab239946 in the following tested applications.
|IP||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 60 kDa.|
FunctionMolecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.
Sequence similaritiesBelongs to the TCP-1 chaperonin family.
Cellular localizationCytoplasm. Cytoplasm > cytoskeleton > centrosome.
- Information by UniProt
- AI528772 antibody
- c-cpn antibody
- CCT alpha antibody
ab109126 at 1:50 dilution (5μg in 1mg lysate) immunoprecipitating TCP1 alpha/CCTA in ECV-304 (human urinary bladder cancer epithelial cell) whole cell lysate.
Lane 1: ECV-304 (human urinary bladder cancer epithelial cell) whole cell lysate
Lane 2: PBS instead of ab109126 in ECV-304 whole cell lysate
For western blotting, Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG was used as the secondary antibody at 1:1500 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109126).
ab239946 has not yet been referenced specifically in any publications.