Overview

  • Product name

  • Description

    Rabbit polyclonal to TCP1 delta
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Sheep, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Common marmoset, Orangutan
  • Immunogen

    Synthetic peptide within Human TCP1 delta aa 489-539 (C terminal). The exact sequence is proprietary. NP_006421.2.
    Sequence:

    KTAGINVRKGGISNILEELVVQPLLVSVSALTLATETVRSILKIDDVVNT R


    Database link: P50991

  • Positive control

    • HeLa, 293T, Jurkat, TCMK1 and NIH 3T3 whole cell lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7-8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab205013 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 58 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Relevance

    The chaperonin containing TCP1 complex (CCT), also called the TCP1 ring complex, consists of 2 back to back rings, each containing 8 unique but homologous subunits (e.g. CCT3). CCT assists the folding of newly translated polypeptide substrates through multiple rounds of ATP driven release and rebinding of partially folded intermediate forms. Substrates of CCT include the cytoskeletal proteins actin and tubulin, as well as alpha transducin. TCP1 delta belongs to the TCP1 chaperonin family, and is a molecular chaperone; assisting the folding of proteins upon ATP hydrolysis. It is known to play a role, in vitro, in the folding of actin and tubulin.
  • Cellular localization

    Cytoplasmic > Cytoskeleton
  • Database links

  • Alternative names

    • CCT delta antibody
    • CCT4 antibody
    • CCTD antibody
    • chaperonin containing t-complex polypeptide 1, delta subunit antibody
    • chaperonin containing TCP1, subunit 4 (delta) antibody
    • SRB antibody
    • Stimulator of TAR RNA binding antibody
    • T complex protein 1 subunit delta antibody
    • TCP-1-delta antibody
    see all

Images

  • Detection of TCP1 delta in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab205013 at 6 µg/mg lysate for IP (Lane 1) and at 0.4 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.

  • All lanes : Anti-TCP1 delta antibody (ab205013) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : 293T whole cell lysate
    Lane 3 : Jurkat whole cell lysate
    Lane 4 : TCMK1 whole cell lysate
    Lane 5 : NIH 3T3 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 58 kDa


    Exposure time: 10 seconds


    Lysates prepared using NETN lysis buffer.

References

ab205013 has not yet been referenced specifically in any publications.

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