Product nameAnti-TCP1 delta antibody
See all TCP1 delta primary antibodies
DescriptionRabbit polyclonal to TCP1 delta
Tested applicationsSuitable for: WB, ELISA, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Saccharomyces cerevisiae, Drosophila melanogaster, Zebrafish
- HepG2 cell lysates
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab49151 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1.25 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 60 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
RelevanceThe chaperonin containing TCP1 complex (CCT), also called the TCP1 ring complex, consists of 2 back to back rings, each containing 8 unique but homologous subunits (e.g. CCT3). CCT assists the folding of newly translated polypeptide substrates through multiple rounds of ATP driven release and rebinding of partially folded intermediate forms. Substrates of CCT include the cytoskeletal proteins actin and tubulin, as well as alpha transducin. TCP1 delta belongs to the TCP1 chaperonin family, and is a molecular chaperone; assisting the folding of proteins upon ATP hydrolysis. It is known to play a role, in vitro, in the folding of actin and tubulin.
Cellular localizationCytoplasmic > Cytoskeleton
- CCT delta antibody
- CCT4 antibody
- CCTD antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bronchial epithelial tissue labelling TCP1 delta with ab49151 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the cytoplasm. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - TCP1. Right - Merge.
Western blot analysis of HepG2 cell lysate labeling TCP1 delta with ab49151 at 1µg/ml.
Immunocytochemistry/immunofluorescence analysis of Rat hippocampal neurons - 14DIV labeling TCP1 delta with ab49151 at 1/200. A Cy3-conjugated anti rabbit IgG (1/500) was used as the secondary antibody.
Western blot analysis of HepG2 cell lysate labeling TCP1 delta with ab49151 at 1:1000. A Cy3-conjugated anti-rabbit IgG (1:10000) was used as the secondary antibody.
All lanes : Anti-TCP1 delta antibody (ab49151) at 1.25 µg/ml (in 5% skim milk / PBS buffer)
Lane 1 : molecular marker
Lane 2 : HepG2 cell lysate at 10 µg
All lanes : HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 60 kDa
Observed band size: 56 kDa why is the actual band size different from the predicted?
Gel concentration: 12%
This product has been referenced in:
- Mallawaaratchy DM et al. The phosphoinositide 3-kinase inhibitor LY294002, decreases aminoacyl-tRNA synthetases, chaperones and glycolytic enzymes in human HT-29 colorectal cancer cells. J Proteomics 75:1590-9 (2012). WB ; Human . Read more (PubMed: 22172953) »