• Product name

  • Description

    Rabbit polyclonal to TCP1 delta
  • Host species

  • Tested applications

    Suitable for: WB, ELISA, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Saccharomyces cerevisiae, Drosophila melanogaster, Zebrafish
  • Immunogen

    Synthetic peptide: AGLNPISTVT ELRNRHAQGE KTAGINVRKG GISNILEELV VQPLLVSVSA, corresponding to a region within C terminal amino acids 469-518 of Human TCP1 delta

  • Positive control

    • HepG2 whole cell lysate (ab7900)


  • Form

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab49151 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 60 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/312500.
ICC/IF 1/200.
IHC-P 1/100.


  • Relevance

    The chaperonin containing TCP1 complex (CCT), also called the TCP1 ring complex, consists of 2 back to back rings, each containing 8 unique but homologous subunits (e.g. CCT3). CCT assists the folding of newly translated polypeptide substrates through multiple rounds of ATP driven release and rebinding of partially folded intermediate forms. Substrates of CCT include the cytoskeletal proteins actin and tubulin, as well as alpha transducin. TCP1 delta belongs to the TCP1 chaperonin family, and is a molecular chaperone; assisting the folding of proteins upon ATP hydrolysis. It is known to play a role, in vitro, in the folding of actin and tubulin.
  • Cellular localization

    Cytoplasmic > Cytoskeleton
  • Database links

  • Alternative names

    • CCT delta antibody
    • CCT4 antibody
    • CCTD antibody
    • chaperonin containing t-complex polypeptide 1, delta subunit antibody
    • chaperonin containing TCP1, subunit 4 (delta) antibody
    • SRB antibody
    • Stimulator of TAR RNA binding antibody
    • T complex protein 1 subunit delta antibody
    • TCP-1-delta antibody
    see all


  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bronchial epithelial tissue labelling TCP1 delta with ab49151 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the cytoplasm. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - TCP1. Right - Merge.
  • Western blot analysis of HepG2 cell lysate labeling TCP1 delta with ab49151 at 1µg/ml.
  • Immunocytochemistry/immunofluorescence analysis of Rat hippocampal neurons - 14DIV labeling TCP1 delta with ab49151 at 1/200. A Cy3-conjugated anti rabbit IgG (1/500) was used as the secondary antibody.
  • Western blot analysis of HepG2 cell lysate labeling TCP1 delta with ab49151 at 1:1000. A Cy3-conjugated anti-rabbit IgG (1:10000) was used as the secondary antibody.
  • All lanes : Anti-TCP1 delta antibody (ab49151) at 1.25 µg/ml (in 5% skim milk / PBS buffer)

    Lane 1 : molecular marker
    Lane 2 : HepG2 cell lysate at 10 µg

    All lanes : HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 60 kDa
    Observed band size: 56 kDa
    why is the actual band size different from the predicted?

    Gel concentration: 12%


This product has been referenced in:

  • Mallawaaratchy DM  et al. The phosphoinositide 3-kinase inhibitor LY294002, decreases aminoacyl-tRNA synthetases, chaperones and glycolytic enzymes in human HT-29 colorectal cancer cells. J Proteomics 75:1590-9 (2012). WB ; Human . Read more (PubMed: 22172953) »
See 1 Publication for this product

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