RecombinantRabMAb

Recombinant Anti-TCP1 delta antibody [EPR8495(B)] (ab129072)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8495(B)] to TCP1 delta
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-TCP1 delta antibody [EPR8495(B)]
    See all TCP1 delta primary antibodies

  • Description

    Rabbit monoclonal [EPR8495(B)] to TCP1 delta

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human TCP1 delta aa 450-550. The exact sequence is proprietary.

  • Positive control

    • HeLa, Jurkat, Ramos and HepG2 whole cell lysate (ab7900); Human stomach tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR8495(B)

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab129072 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.

    • Target

    • Relevance

      The chaperonin containing TCP1 complex (CCT), also called the TCP1 ring complex, consists of 2 back to back rings, each containing 8 unique but homologous subunits (e.g. CCT3). CCT assists the folding of newly translated polypeptide substrates through multiple rounds of ATP driven release and rebinding of partially folded intermediate forms. Substrates of CCT include the cytoskeletal proteins actin and tubulin, as well as alpha transducin. TCP1 delta belongs to the TCP1 chaperonin family, and is a molecular chaperone; assisting the folding of proteins upon ATP hydrolysis. It is known to play a role, in vitro, in the folding of actin and tubulin.

    • Cellular localization

      Cytoplasmic > Cytoskeleton

    • Database links

      see all

    • Alternative names

      • CCT delta antibody
      • CCT4 antibody
      • CCTD antibody
      • chaperonin containing t-complex polypeptide 1, delta subunit antibody
      • chaperonin containing TCP1, subunit 4 (delta) antibody
      • SRB antibody
      • Stimulator of TAR RNA binding antibody
      • T complex protein 1 subunit delta antibody
      • TCP-1-delta antibody
      see all

    Images

    • Western blot - Anti-TCP1 delta antibody [EPR8495(B)] (ab129072)
      Western blot - Anti-TCP1 delta antibody [EPR8495(B)] (ab129072)
      All lanes : Anti-TCP1 delta antibody [EPR8495(B)] (ab129072) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : Ramos cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 58 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 delta antibody [EPR8495(B)] (ab129072)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 delta antibody [EPR8495(B)] (ab129072)

      ab129072, at 1/50 dilution, staining TCP1 delta in paraffin-embedded Human stomach tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Anti-TCP1 delta antibody [EPR8495(B)] (ab129072)
      Anti-TCP1 delta antibody [EPR8495(B)] (ab129072)

    References (1)

    Publishing research using ab129072? Please let us know so that we can cite the reference in this datasheet.

    ab129072 has been referenced in 1 publication.

    • Showalter AE  et al. Investigating Chaperonin-Containing TCP-1 subunit 2 as an essential component of the chaperonin complex for tumorigenesis. Sci Rep 10:798 (2020). PubMed: 31964905

    Customer reviews and Q&As

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    Western blot abreview for Anti-TCP1 delta antibody [EPR8495(B)]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (primary embryonic rat cortical neuron)
    Gel Running Conditions
    Reduced Denaturing (10% SDS-PAGE)
    Loading amount
    200000 cells
    Treatment
    Lentivirus mediated knockdown of CCT2 induced down regulation of CCT4 in primary rat cortical neurons
    Specification
    primary embryonic rat cortical neuron
    Blocking step
    Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 26 2020

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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