Recombinant Anti-TCP1 delta antibody [EPR8495(B)] (ab129072)
- Datasheet
- References
- Protocols
Overview
-
Product name
Anti-TCP1 delta antibody [EPR8495(B)]
See all TCP1 delta primary antibodies -
Description
Rabbit monoclonal [EPR8495(B)] to TCP1 delta -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human TCP1 delta aa 450-550. The exact sequence is proprietary.
-
Positive control
- HeLa, Jurkat, Ramos and HepG2 whole cell lysate (ab7900); Human stomach tissue.
-
General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8495(B) -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
Applications
Our Abpromise guarantee covers the use of ab129072 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa). | |
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
-
Relevance
The chaperonin containing TCP1 complex (CCT), also called the TCP1 ring complex, consists of 2 back to back rings, each containing 8 unique but homologous subunits (e.g. CCT3). CCT assists the folding of newly translated polypeptide substrates through multiple rounds of ATP driven release and rebinding of partially folded intermediate forms. Substrates of CCT include the cytoskeletal proteins actin and tubulin, as well as alpha transducin. TCP1 delta belongs to the TCP1 chaperonin family, and is a molecular chaperone; assisting the folding of proteins upon ATP hydrolysis. It is known to play a role, in vitro, in the folding of actin and tubulin. -
Cellular localization
Cytoplasmic > Cytoskeleton -
Database links
- Entrez Gene: 10575 Human
- Entrez Gene: 12464 Mouse
- Entrez Gene: 29374 Rat
- Omim: 605142 Human
- SwissProt: P50991 Human
- SwissProt: P80315 Mouse
- SwissProt: Q7TPB1 Rat
- Unigene: 421509 Human
see all -
Alternative names
- CCT delta antibody
- CCT4 antibody
- CCTD antibody
see all
Images
-
![Western blot - Anti-TCP1 delta antibody [EPR8495(B)] (ab129072)](https://www.abcam.com/ps/products/129/ab129072/Images/ab129072-143017-anti-tcp1-delta-antibody-epr8495b-western-blot.jpg)
Western blot - Anti-TCP1 delta antibody [EPR8495(B)] (ab129072)All lanes : Anti-TCP1 delta antibody [EPR8495(B)] (ab129072) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : Ramos cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 58 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 delta antibody [EPR8495(B)] (ab129072)](https://www.abcam.com/ps/products/129/ab129072/Images/ab129072-143016-anti-tcp1-delta-antibody-epr8495b-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 delta antibody [EPR8495(B)] (ab129072)ab129072, at 1/50 dilution, staining TCP1 delta in paraffin-embedded Human stomach tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Datasheets and documents
References
ab129072 has not yet been referenced specifically in any publications.
