Overview

  • Product name

    Anti-TCP1 epsilon/CCT5 antibody
    See all TCP1 epsilon/CCT5 primary antibodies
  • Description

    Rabbit polyclonal to TCP1 epsilon/CCT5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Orangutan
  • Immunogen

    Synthetic peptide within Human TCP1 epsilon/CCT5 aa 100-150. The exact sequence is proprietary. (NP_036205.1).
    Database link: P48643

  • Positive control

    • IHC-P: Human prostate carcinoma tissue; Mouse teratoma tissue. WB: HEK-293T, Jurkat, HeLa and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate.
  • General notes

     This product was previously labelled as TCP1 epsilon

     

Properties

Applications

Our Abpromise guarantee covers the use of ab225876 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 60 kDa.
IP Use at 2-10 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.
  • Involvement in disease

    Neuropathy, hereditary sensory, with spastic paraplegia, autosomal recessive
  • Sequence similarities

    Belongs to the TCP-1 chaperonin family.
  • Cellular localization

    Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
  • Information by UniProt
  • Database links

  • Alternative names

    • CCT 5 antibody
    • CCT epsilon antibody
    • CCT-epsilon antibody
    • CCT5 antibody
    • CCTE antibody
    • CCTepsilon antibody
    • Chaperonin containing T-complex polypeptide 1, subunit 5 antibody
    • Chaperonin containing TCP 1 epsilon antibody
    • Chaperonin containing TCP 1 subunit 5 antibody
    • Chaperonin containing TCP 1 subunit 5 epsilon antibody
    • Chaperonin containing TCP1 epsilon antibody
    • Chaperonin containing TCP1 subunit 5 antibody
    • Chaperonin containing TCP1 subunit 5 epsilon antibody
    • KIAA0098 antibody
    • T complex protein 1 epsilon subunit antibody
    • T complex protein 1 subunit epsilon antibody
    • T-complex protein 1 subunit epsilon antibody
    • TCP 1 epsilon antibody
    • TCP-1-epsilon antibody
    • TCP1epsilon antibody
    • TCPE antibody
    • TCPE_HUMAN antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for TCP1 epsilon/CCT5 using ab225876 at 1/200 dilution in immunohistochemical analysis. Detection: DAB staining. Counterstain: hematoxylin (blue).

  • All lanes : Anti-TCP1 epsilon/CCT5 antibody (ab225876) at 0.04 µg/ml

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
    Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
    Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 60 kDa


    Exposure time: 3 seconds
  • TCP1 epsilon/CCT5 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225876 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab225876 at 0.4 µg/ml.

    Lane 1: ab225876 IP in HEK-293T whole cell lysate.

    Lane 2: Control IgG IP in HEK-293T whole cell lysate.

    Detection:  Chemiluminescence with exposure time of 1 second.

  • Formalin-fixed, paraffin-embedded mouse teratoma tissue stained for TCP1 epsilon/CCT5 using ab225876 at 1/200 dilution in immunohistochemical analysis. Detection: DAB staining. Counterstain: hematoxylin (blue).

References

ab225876 has not yet been referenced specifically in any publications.

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