Key features and details
- Rabbit polyclonal to TCP1 epsilon/CCT5
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-TCP1 epsilon/CCT5 antibody
See all TCP1 epsilon/CCT5 primary antibodies
DescriptionRabbit polyclonal to TCP1 epsilon/CCT5
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Orangutan
Synthetic peptide within Human TCP1 epsilon/CCT5 aa 100-150. The exact sequence is proprietary. (NP_036205.1).
Database link: P48643
- IHC-P: Human prostate carcinoma tissue; Mouse teratoma tissue. WB: HEK-293T, Jurkat, HeLa and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate.
This product was previously labelled as TCP1 epsilon
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab225876 was affinity purified using an epitope specific to TCP1 epsilon/CCT5 immobilized on solid support.
Our Abpromise guarantee covers the use of ab225876 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 60 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionMolecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.
Involvement in diseaseNeuropathy, hereditary sensory, with spastic paraplegia, autosomal recessive
Sequence similaritiesBelongs to the TCP-1 chaperonin family.
Cellular localizationCytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
- Information by UniProt
- CCT 5 antibody
- CCT epsilon antibody
- CCT-epsilon antibody
Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for TCP1 epsilon/CCT5 using ab225876 at 1/200 dilution in immunohistochemical analysis. Detection: DAB staining. Counterstain: hematoxylin (blue).
All lanes : Anti-TCP1 epsilon/CCT5 antibody (ab225876) at 0.04 µg/ml
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 60 kDa
Exposure time: 3 seconds
TCP1 epsilon/CCT5 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225876 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab225876 at 0.4 µg/ml.
Lane 1: ab225876 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 1 second.
Formalin-fixed, paraffin-embedded mouse teratoma tissue stained for TCP1 epsilon/CCT5 using ab225876 at 1/200 dilution in immunohistochemical analysis. Detection: DAB staining. Counterstain: hematoxylin (blue).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab225876 has not yet been referenced specifically in any publications.