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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Actin Assembly
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Anti-TCP1 epsilon/CCT5 antibody (ab96344)

  • Datasheet
  • SDS
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Western blot - Anti-TCP1 epsilon/CCT5 antibody (ab96344)
  • Immunocytochemistry/ Immunofluorescence - Anti-TCP1 epsilon/CCT5 antibody (ab96344)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 epsilon/CCT5 antibody (ab96344)

Key features and details

  • Rabbit polyclonal to TCP1 epsilon/CCT5
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human TCP1 epsilon/CCT5 protein (ab132283)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-TCP1 epsilon/CCT5 antibody
    See all TCP1 epsilon/CCT5 primary antibodies
  • Description

    Rabbit polyclonal to TCP1 epsilon/CCT5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment corresponding to Human TCP1 epsilon/CCT5 aa 89-308.
    Database link: NP_036205

  • Positive control

    • Molt-4 whole cell lysate; HeLa cells; NCIN87 xenograft; 293T, A431, H1299, HepG2 and Raji cell lines
  • General notes

     This product was previously labelled as TCP1 epsilon

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Assembly
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microtubules
    • Tubulin
    • Signal Transduction
    • Protein Trafficking
    • Chaperones
    • Other Chaperones

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • MOLT4 whole cell lysate (ab7912)
  • Recombinant Protein

    • Recombinant Human TCP1 epsilon/CCT5 protein (ab132283)

Applications

Our Abpromise guarantee covers the use of ab96344 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500.
WB 1/500 - 1/3000. Predicted molecular weight: 60 kDa.
ICC/IF 1/100 - 1/200.

Target

  • Function

    Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.
  • Involvement in disease

    Neuropathy, hereditary sensory, with spastic paraplegia, autosomal recessive
  • Sequence similarities

    Belongs to the TCP-1 chaperonin family.
  • Cellular localization

    Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
  • Target information above from: UniProt accession P48643 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 533784 Cow
    • Entrez Gene: 22948 Human
    • Entrez Gene: 12465 Mouse
    • Entrez Gene: 294864 Rat
    • Omim: 610150 Human
    • SwissProt: P48643 Human
    • SwissProt: P80316 Mouse
    • SwissProt: Q68FQ0 Rat
    • Unigene: 1600 Human
    • Unigene: 740762 Human
    • Unigene: 282158 Mouse
    • Unigene: 92645 Rat
    see all
  • Alternative names

    • CCT 5 antibody
    • CCT epsilon antibody
    • CCT-epsilon antibody
    • CCT5 antibody
    • CCTE antibody
    • CCTepsilon antibody
    • Chaperonin containing T-complex polypeptide 1, subunit 5 antibody
    • Chaperonin containing TCP 1 epsilon antibody
    • Chaperonin containing TCP 1 subunit 5 antibody
    • Chaperonin containing TCP 1 subunit 5 epsilon antibody
    • Chaperonin containing TCP1 epsilon antibody
    • Chaperonin containing TCP1 subunit 5 antibody
    • Chaperonin containing TCP1 subunit 5 epsilon antibody
    • KIAA0098 antibody
    • T complex protein 1 epsilon subunit antibody
    • T complex protein 1 subunit epsilon antibody
    • T-complex protein 1 subunit epsilon antibody
    • TCP 1 epsilon antibody
    • TCP-1-epsilon antibody
    • TCP1epsilon antibody
    • TCPE antibody
    • TCPE_HUMAN antibody
    see all

Images

  • Western blot - Anti-TCP1 epsilon/CCT5 antibody (ab96344)
    Western blot - Anti-TCP1 epsilon/CCT5 antibody (ab96344)
    Anti-TCP1 epsilon/CCT5 antibody (ab96344) at 1/1000 dilution + Molt-4 whole cell lysate at 30 µg

    Predicted band size: 60 kDa



    7.5% SDS PAGE
  • Immunocytochemistry/ Immunofluorescence - Anti-TCP1 epsilon/CCT5 antibody (ab96344)
    Immunocytochemistry/ Immunofluorescence - Anti-TCP1 epsilon/CCT5 antibody (ab96344)
    Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab96344 at 1/200 dilution. Lower image is merged with DNA probe.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 epsilon/CCT5 antibody (ab96344)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 epsilon/CCT5 antibody (ab96344)
    Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using ab96344 at 1/500 dilution.

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab96344? Please let us know so that we can cite the reference in this datasheet.

    ab96344 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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