Anti-TCP1 epsilon/CCT5 antibody (ab96344)
- Datasheet
- References
- Protocols
Overview
-
Product name
Anti-TCP1 epsilon/CCT5 antibody
See all TCP1 epsilon/CCT5 primary antibodies -
Description
Rabbit polyclonal to TCP1 epsilon/CCT5 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow
-
Immunogen
Recombinant fragment corresponding to Human TCP1 epsilon/CCT5 aa 89-308.
Database link: NP_036205 -
Positive control
- Molt-4 whole cell lysate; HeLa cells; NCIN87 xenograft; 293T, A431, H1299, HepG2 and Raji cell lines
-
General notes
This product was previously labelled as TCP1 epsilon
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab96344 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100 - 1/500. | |
| WB | 1/500 - 1/3000. Predicted molecular weight: 60 kDa. | |
| ICC/IF | 1/100 - 1/200. |
Target
-
Function
Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin. -
Involvement in disease
Neuropathy, hereditary sensory, with spastic paraplegia, autosomal recessive -
Sequence similarities
Belongs to the TCP-1 chaperonin family. -
Cellular localization
Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. - Information by UniProt
-
Database links
- Entrez Gene: 533784 Cow
- Entrez Gene: 22948 Human
- Entrez Gene: 12465 Mouse
- Entrez Gene: 294864 Rat
- Omim: 610150 Human
- SwissProt: P48643 Human
- SwissProt: P80316 Mouse
- SwissProt: Q68FQ0 Rat
see all -
Alternative names
- CCT 5 antibody
- CCT epsilon antibody
- CCT-epsilon antibody
see all
Images
-
Western blot - Anti-TCP1 epsilon/CCT5 antibody (ab96344)Anti-TCP1 epsilon/CCT5 antibody (ab96344) at 1/1000 dilution + Molt-4 whole cell lysate at 30 µg
Predicted band size: 60 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-TCP1 epsilon/CCT5 antibody (ab96344)Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab96344 at 1/200 dilution. Lower image is merged with DNA probe. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 epsilon/CCT5 antibody (ab96344)Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using ab96344 at 1/500 dilution.
Protocols
References
ab96344 has not yet been referenced specifically in any publications.
