Key features and details
- Rabbit polyclonal to TCP1 epsilon/CCT5
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TCP1 epsilon/CCT5 antibody
See all TCP1 epsilon/CCT5 primary antibodies
DescriptionRabbit polyclonal to TCP1 epsilon/CCT5
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
Recombinant fragment corresponding to Human TCP1 epsilon/CCT5 aa 89-308.
Database link: NP_036205
- Molt-4 whole cell lysate; HeLa cells; NCIN87 xenograft; 293T, A431, H1299, HepG2 and Raji cell lines
This product was previously labelled as TCP1 epsilon
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab96344 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500.|
|WB||1/500 - 1/3000. Predicted molecular weight: 60 kDa.|
|ICC/IF||1/100 - 1/200.|
FunctionMolecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.
Involvement in diseaseNeuropathy, hereditary sensory, with spastic paraplegia, autosomal recessive
Sequence similaritiesBelongs to the TCP-1 chaperonin family.
Cellular localizationCytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
- Information by UniProt
- CCT 5 antibody
- CCT epsilon antibody
- CCT-epsilon antibody
Anti-TCP1 epsilon/CCT5 antibody (ab96344) at 1/1000 dilution + Molt-4 whole cell lysate at 30 µg
Predicted band size: 60 kDa
7.5% SDS PAGE
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab96344 at 1/200 dilution. Lower image is merged with DNA probe.
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using ab96344 at 1/500 dilution.
ab96344 has not yet been referenced specifically in any publications.