Anti-TCP1 epsilon/CCT5 antibody (ab96344)
Key features and details
- Rabbit polyclonal to TCP1 epsilon/CCT5
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TCP1 epsilon/CCT5 antibody
See all TCP1 epsilon/CCT5 primary antibodies -
Description
Rabbit polyclonal to TCP1 epsilon/CCT5 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow
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Immunogen
Recombinant fragment corresponding to Human TCP1 epsilon/CCT5 aa 89-308.
Database link: NP_036205 -
Positive control
- Molt-4 whole cell lysate; HeLa cells; NCIN87 xenograft; 293T, A431, H1299, HepG2 and Raji cell lines
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General notes
This product was previously labelled as TCP1 epsilon
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab96344 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100 - 1/500. | |
| WB | 1/500 - 1/3000. Predicted molecular weight: 60 kDa. | |
| ICC/IF | 1/100 - 1/200. |
Target
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Function
Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin. -
Involvement in disease
Neuropathy, hereditary sensory, with spastic paraplegia, autosomal recessive -
Sequence similarities
Belongs to the TCP-1 chaperonin family. -
Cellular localization
Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. - Information by UniProt
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Database links
- Entrez Gene: 533784 Cow
- Entrez Gene: 22948 Human
- Entrez Gene: 12465 Mouse
- Entrez Gene: 294864 Rat
- Omim: 610150 Human
- SwissProt: P48643 Human
- SwissProt: P80316 Mouse
- SwissProt: Q68FQ0 Rat
see all -
Alternative names
- CCT 5 antibody
- CCT epsilon antibody
- CCT-epsilon antibody
see all
Images
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Western blot - Anti-TCP1 epsilon/CCT5 antibody (ab96344)Anti-TCP1 epsilon/CCT5 antibody (ab96344) at 1/1000 dilution + Molt-4 whole cell lysate at 30 µg
Predicted band size: 60 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-TCP1 epsilon/CCT5 antibody (ab96344)Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab96344 at 1/200 dilution. Lower image is merged with DNA probe. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 epsilon/CCT5 antibody (ab96344)Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using ab96344 at 1/500 dilution.
Protocols
References (0)
ab96344 has not yet been referenced specifically in any publications.
