Key features and details
- Rabbit polyclonal to TCP1 eta
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TCP1 eta antibody
See all TCP1 eta primary antibodies
DescriptionRabbit polyclonal to TCP1 eta
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Cow, Orangutan
Recombinant fragment corresponding to Human TCP1 eta aa 240-543.
LNVELELKAEKDNAEIRVHTVEDYQAIVDAEWNILYDKLEKIHHSGAKVV LSKLPIGDVATQYFADRDMFCAGRVPEEDLKRTMMACGGSIQTSVNALSA DVLGRCQVFEETQIGGERYNFFTGCPKAKTCTFILRGGAEQFMEETERSL HDAIMIVRRAIKNDSVVAGGGAIEMELSKYLRDYSRTIPGKQQLLIGAYA KALEIIPRQLCDNAGFDATNILNKLRARHAQGGTWYGVDINNEDIADNFE AFVWEPAMVRINALTAASEAACLIVSVDETIKNPRSTVDAPTAAGRGRGR GRPH
Database link: Q99832-1
- WB: K562 and HEK-293 whole cell lysates. IHC-P: Human glioma tissue. ICC/IF: MCF7 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity greater than 95%.
Our Abpromise guarantee covers the use of ab237762 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/200.|
|IHC-P||1/20 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/500 - 1/5000. Predicted molecular weight: 59 kDa.|
FunctionMolecular chaperone; assists the folding of proteins upon ATP hydrolysis. Known to play a role, in vitro, in the folding of actin and tubulin.
Sequence similaritiesBelongs to the TCP-1 chaperonin family.
- Information by UniProt
- CCT ETA antibody
- CCT-eta antibody
- CCT7 antibody
All lanes : Anti-TCP1 eta antibody (ab237762) at 1/500 dilution
Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 59 kDa
Paraffin-embedded human glioma tissue stained for TCP1 eta with ab237762 at 1/100 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
MCF7 (human breast adenocarcinoma cell line) cells stained for TCP1 eta using ab237762 at 1/100 dilution in ICC/IF.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the primary antibody overnight at 4°C. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L). Counterstained with DAPI.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab237762 has not yet been referenced specifically in any publications.