Recombinant Anti-TCP1 eta antibody [EPR10944(B)] (ab166891)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-TCP1 eta antibody [EPR10944(B)]
See all TCP1 eta primary antibodies -
Description
Rabbit monoclonal [EPR10944(B)] to TCP1 eta -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to residues in Human TCP1 eta (UniProt: Q99832).
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Positive control
- HeLa, 293T, K562 and A431 cell lysates; Human cervical carcinoma tissue, HeLa cells
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10944(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab166891 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000 - 1/50000. Predicted molecular weight: 59 kDa. | |
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | 1/50 - 1/100. |
Target
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Function
Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. Known to play a role, in vitro, in the folding of actin and tubulin. -
Sequence similarities
Belongs to the TCP-1 chaperonin family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 10574 Human
- Omim: 605140 Human
- SwissProt: Q99832 Human
- Unigene: 368149 Human
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Alternative names
- CCT ETA antibody
- CCT-eta antibody
- CCT7 antibody
see all
Images
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![Western blot - Anti-TCP1 eta antibody [EPR10944(B)] (ab166891)](https://www.abcam.com/ps/products/166/ab166891/Images/ab166891-165228-anti-tcp1-eta-antibody-epr10944b-western-blot.jpg)
Western blot - Anti-TCP1 eta antibody [EPR10944(B)] (ab166891)All lanes : Anti-TCP1 eta antibody [EPR10944(B)] (ab166891) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : K562 cell lysate
Lane 4 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 59 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 eta antibody [EPR10944(B)] (ab166891)](https://www.abcam.com/ps/products/166/ab166891/Images/ab166891-165227-anti-tcp1-eta-antibody-epr10944b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 eta antibody [EPR10944(B)] (ab166891)Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue labeling TCP1 eta with ab166891 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-TCP1 eta antibody [EPR10944(B)] (ab166891)](https://www.abcam.com/ps/products/166/ab166891/Images/ab166891-165226-anti-tcp1-eta-antibody-epr10944b-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-TCP1 eta antibody [EPR10944(B)] (ab166891)Immunofluorescent analysis of HeLa cells labeling TCP1 eta with ab166891 at 1/50 dilution.
Protocols
Datasheets and documents
References
ab166891 has not yet been referenced specifically in any publications.
