Key features and details
- Rabbit polyclonal to TCP1 theta
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-TCP1 theta antibody
See all TCP1 theta primary antibodies
DescriptionRabbit polyclonal to TCP1 theta
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Chicken, Cow, Cynomolgus monkey, Orangutan
- HeLa, 293T, Jurkat or Mouse NIH3T3 lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, 99% Tris buffered saline
pH: 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab176691 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 60 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
RelevanceTCP1 theta is the theta polypeptide of the CCT chaperonin molecule complex. The intact CCT complex is composed of eight polypeptides in a double ring. TCP1 theta is a molecular chaperone that assists the folding of proteins upon ATP hydrolysis. CCT is important within cells in aiding the folding of proteins including actin, tubulin and the VHL tumour suppressor protein.
Cellular localizationCytoplasm. Cytoplasm; cytoskeleton; centrosome
- C21orf112 antibody
- CCT 8 antibody
- CCT theta antibody
Detection of Human TCP1 theta by Western Blot of Immunoprecipitates. 1 mg for IP; 20% of IP HeLa whole cell lysate loaded. ab176691 used for IP at 6 µg/mg lysate. For blotting immunoprecipitated TCP1 theta, ab176691 was used at 0.04 µg/ml.
All lanes : Anti-TCP1 theta antibody (ab176691) at 0.04 µg/ml
Lane 1 : HeLa lysate at 50 µg
Lane 2 : HeLa lysate at 15 µg
Lane 3 : 293T lysate at 50 µg
Lane 4 : Jurkat lysate at 50 µg
Lane 5 : Mouse NIH3T3 lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 60 kDa
Exposure time: 10 seconds
ab176691 has not yet been referenced specifically in any publications.