Overview

  • Product name

  • Description

    Rabbit polyclonal to TCP1 theta
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Chimpanzee, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human TCP1 theta (internal sequence). The exact sequence is proprietary.
    Database link: P50990

  • Positive control

    • WB: Raji whole cell lysate. ICC/IF: A431 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: PBS, 1% BSA, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab228746 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000. Predicted molecular weight: 60 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Relevance

    TCP1 theta is the theta polypeptide of the CCT chaperonin molecule complex. The intact CCT complex is composed of eight polypeptides in a double ring. TCP1 theta is a molecular chaperone that assists the folding of proteins upon ATP hydrolysis. CCT is important within cells in aiding the folding of proteins including actin, tubulin and the VHL tumour suppressor protein.
  • Cellular localization

    Cytoplasm. Cytoplasm; cytoskeleton; centrosome
  • Database links

  • Alternative names

    • C21orf112 antibody
    • CCT 8 antibody
    • CCT theta antibody
    • CCT8 antibody
    • CCTQ antibody
    • Chaperonin containing TCP1 subunit 8 (theta) antibody
    • Chaperonin subunit 8 (Theta) (Predicted), isoform CRA_a antibody
    • Chaperonin subunit 8 (theta) antibody
    • Chromosome 21 open reading frame 112 antibody
    • D21S246 antibody
    • KIAA0002 antibody
    • PRED71 antibody
    • Renal carcinoma antigen NY REN 15 antibody
    • T complex protein 1 subunit theta antibody
    • T complex protein 1 theta subunit antibody
    • TCP 1 theta antibody
    • Tcpq antibody
    see all

Images

  • Anti-TCP1 theta antibody (ab228746) at 1/10000 dilution + Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg

    Predicted band size: 60 kDa



    10% SDS-PAGE gel.

  • Methanol-fixed A431 (human epidermoid carcinoma cell line) cells stained for TCP1 theta (green) using ab228746 at 1/500 dilution in ICC/IF.

    Nuclear counterstain: Hoechst 33342 (blue).

References

ab228746 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab228746.
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