Overview

  • Product name
  • Description
    Rabbit polyclonal to TCPTP
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Recombinant fragment
    Predicted to work with: Human
  • Immunogen

    Two separate synthetic peptides conjugated to KLH derived from within residues 300 - 400 of Human TCPTP.

    Read Abcam's proprietary immunogen policy (Peptides available as ab14809 and ab14810.)

  • Positive control
    • This antibody recognises the recombinant TCPTP fusion protein (60kDa)in Western blots.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS. pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab1996 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 48 kDa.

Target

  • Tissue specificity
    Isoform PTPA is probably the major isoform. Isoform PTPB is expressed in T-cells and in placenta.
  • Sequence similarities
    Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.
    Contains 1 tyrosine-protein phosphatase domain.
  • Cellular localization
    Cytoplasm; Nucleus and Endoplasmic reticulum.
  • Information by UniProt
  • Database links
  • Alternative names
    • Protein tyrosine phosphatase non receptor type 2 antibody
    • PTN2 antibody
    • PTN2_HUMAN antibody
    • PTPN 2 antibody
    • Ptpn2 antibody
    • PTPT antibody
    • T cell protein tyrosine phosphatase antibody
    • T-cell protein-tyrosine phosphatase antibody
    • TC PTP antibody
    • TCELLPTP antibody
    • TCPTP antibody
    • Tyrosine protein phosphatase non receptor type 2 antibody
    • Tyrosine-protein phosphatase non-receptor type 2 antibody
    see all

Images

  • All lanes : Anti-TCPTP antibody (ab1996) at 1/2000 dilution

    Lane 1 : PTP1B (PTPN1)
    Lane 2 : TCPTP (PTPN2)
    Lane 3 : PTPH1 (PTPN3)
    Lane 4 : MEG1 (PTPN4)
    Lane 5 : STEP (PTPN5)
    Lane 6 : SHP1 (PTPN6)
    Lane 7 : HePTP (PTPN7)
    Lane 8 : SHP2 (PTPN11)
    Lane 9 : BDP1 (PTPN18)

    Predicted band size: 48 kDa



    Recombinant PTP phosphatase domains were probed with ab1996 at 1/2000.  The antibody recognises the recombinant TCPTP fusion protein at ~60 kDa.

    Lane 1: PTP1B (PTPN1)
    Lane 2:TCPTP (PTPN2)
    Lane 3:PTPH1 (PTPN3)
    Lane 4:MEG1 (PTPN4)
    Lane 5:STEP (PTPN5)
    Lane 6:SHP1 (PTPN6)
    Lane 7:HePTP (PTPN7)
    Lane 8:SHP2 (PTPN11)
    Lane 9:BDP1 (PTPN18)

    Recombinant PTP phosphatase domains were probed with ab1996 at 1/2000. The antibody recognises the recombinant TCPTP fusion protein at ~60 kDa.

References

ab1996 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for your enquiry and for filling the on-line Questionnaire. This antibody has been tested for Western blot application only, and we do not have any information if it is suitable for IP. Unfortunately, we do not have any information on IP. Ab1996 has been characterised for Western blot assay using recombinant TCPTP fusion protein. As the datasheet indicates it is expected to cross-react with Human (100% identity with immunogen) due to sequence homology. We would suggest running purified protein and applying positive control (i.e. human T cell or placenta) along with the samples. Do the endothelial cells express TCPTB at high or low levels? Do you have any preliminary background information? When you prepare the cell lysate, use RIPA buffer rather than standard NP-40 and 2X sample loading buffer which contain a cocktail of different proteinase inhibitors. It is important to use RIPA in order to prevent the degradation of the target protein in the samples. Preparation of RIPA cell lysates for Western Blots: 1. Wash cell pellets once with ice-cold PBS. 2. Add 1 ml of RIPA buffer to 10x8 cells, incubate on ice for 20 min, vortex 2 to 3 times. 3. Centrifuge for 5 min at 4oC at maximum speed in a microfuge tube. 4. Transfer supernatant into clean tube. Measure protein concentration with a protein assay. 5. Adjust concentration to 5 mg/ml with RIPA lysis buffer. 6. Add equal volume of 2 x SDS sample buffer into cell lysate, boil for 5 min. 7. Store at -20oC for daily use or -80oC for long term. Avoid repeated freeze thaw cycles. RIPA Base Ingredients Tris-HCl: 50 mM, pH 7.4 NP-40: 1% Na-deoxycholate: 0.25% NaCl: 150 mM EDTA: 1 mM PMSF: 1 mM Aprotinin, leupeptin, pepstatin: 1 microgram/ml each RIPA Protease Inhibitors Phenylmethylsulfonyl fluoride (PMSF) (200 mM stock solution in isopropanol; store at room temperature) EDTA (calcium chelator; 100 mM stock solution in H2O, pH 7.4) Leupeptin (store frozen in aliquots, 1 mg/ml in H2O) Aprotinin (store frozen in aliquots, 1 mg/ml in H2O) Pepstatin (store frozen in aliquots, 1 mg/ml in methanol) We hope this information will be useful for you.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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