Overview

  • Product name

  • Description

    Rabbit polyclonal to TCPTP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Recombinant fragment within Human TCPTP (internal sequence). The exact sequence is proprietary.
    Database link: P17706

  • Positive control

    • WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts. IP: A431 whole cell extract. IHC-P: Mouse brain tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin
    Constituents: PBS, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab227916 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 48 kDa.
IP 1/100 - 1/571.

Target

Images

  • All lanes : Anti-TCPTP antibody (ab227916) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 48 kDa



    10% SDS-PAGE gel.

  • Paraffin-embedded mouse brain tissue stained for TCPTP using ab227916 at 1/500 dilution in immunohistochemical analysis.

  • TCPTP was immunoprecipitated from A431 (human epidermoid carcinoma cell line) whole cell extract with 5 µg of ab227916. Western blot was performed from the immunoprecipitate using ab227916. Anti-Rabbit IgG was used as a secondary reagent.

    Lane 1: Control IgG instaed of ab227916 in A431 whole cell extract. 

    Lane 2: ab227916 IP in A431 whole cell extract. 

References

ab227916 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab227916.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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