Product nameAnti-TCPTP antibody
See all TCPTP primary antibodies
DescriptionRabbit polyclonal to TCPTP
Tested applicationsSuitable for: IHC-P, WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow
Recombinant fragment within Human TCPTP (internal sequence). The exact sequence is proprietary.
Database link: P17706
- WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts. IP: A431 whole cell extract. IHC-P: Mouse brain tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227916 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 48 kDa.|
|IP||1/100 - 1/571.|
Tissue specificityIsoform PTPA is probably the major isoform. Isoform PTPB is expressed in T-cells and in placenta.
Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.
Contains 1 tyrosine-protein phosphatase domain.
Cellular localizationCytoplasm; Nucleus and Endoplasmic reticulum.
- Information by UniProt
- Protein tyrosine phosphatase non receptor type 2 antibody
- PTN2 antibody
- PTN2_HUMAN antibody
All lanes : Anti-TCPTP antibody (ab227916) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 48 kDa
10% SDS-PAGE gel.
Paraffin-embedded mouse brain tissue stained for TCPTP using ab227916 at 1/500 dilution in immunohistochemical analysis.
TCPTP was immunoprecipitated from A431 (human epidermoid carcinoma cell line) whole cell extract with 5 µg of ab227916. Western blot was performed from the immunoprecipitate using ab227916. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: Control IgG instaed of ab227916 in A431 whole cell extract.
Lane 2: ab227916 IP in A431 whole cell extract.
ab227916 has not yet been referenced specifically in any publications.