Product nameAnti-TCPTP antibody [EPR6712]
See all TCPTP primary antibodies
DescriptionRabbit monoclonal [EPR6712] to TCPTP
Tested applicationsSuitable for: Flow Cyt, WB, IPmore details
Unsuitable for: ICC/IF or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human TCPTP aa 1-100. The exact sequence is proprietary.
- HL60, NIH 3T3, MOLT4 and HepG2 whole cell lysate (ab7900); Mouse placenta and thymus lysates.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab129070 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 45-48 kDa (predicted molecular weight: 48 kDa).|
|IP||1/10 - 1/100.|
Tissue specificityIsoform PTPA is probably the major isoform. Isoform PTPB is expressed in T-cells and in placenta.
Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.
Contains 1 tyrosine-protein phosphatase domain.
Cellular localizationCytoplasm; Nucleus and Endoplasmic reticulum.
- Information by UniProt
- Protein tyrosine phosphatase non receptor type 2 antibody
- PTN2 antibody
- PTN2_HUMAN antibody
All lanes : Anti-TCPTP antibody [EPR6712] (ab129070) at 1/1000 dilution
Lane 1 : HL60 cell lysate
Lane 2 : NIH 3T3 cell lysate
Lane 3 : Mouse placenta lysate
Lane 4 : Mouse thymus lysate
Lane 5 : MOLT4 cell lysate
Lane 6 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 48 kDa
Observed band size: 45-48 kDa why is the actual band size different from the predicted?
Flow cytometric analysis of MOLT-4 (human lymphoblastic leukemia T lymphoblast) cell line fixed with 4% paraformaldehyde and permeabilized with 90% methanol labeling TCPTP with ab129070 at 1:200 dilution (red). This is compared with a Rabbit monoclonal IgG – Isotype control (ab172730) (black) and a unalbelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1:2000 dilution was used as the secondary antibody.
Lane 1 (input): MOLT-4 (human lymphoblastic leukemia T lymphoblast) whole cell lysate, 10μg
Lane 2 (+): MOLT-4 whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab129070 in MOLT-4 whole cell lysate.
Ab129070 Immunoprecipitating TCPTP in MOLT-4 whole cell lysate. Capture antibody was used at a 1:20 dilution (0.6μg in 0.35mg lysates). For western blotting, primary antibody used was ab129070 at 1:1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST
Exposure time: 1second.
ab129070 has not yet been referenced specifically in any publications.