Overview

  • Product name

    Anti-TCPTP antibody - N-terminal
    See all TCPTP primary antibodies
  • Description

    Rabbit polyclonal to TCPTP - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human TCPTP aa 1-50 (N terminal). The exact sequence is proprietary.
    Database link: P17706

  • Positive control

    • WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HeLa whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab226301 was affinity purified using an epitope specific to TCPTP immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab226301 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2500. Predicted molecular weight: 48 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Tissue specificity

    Isoform PTPA is probably the major isoform. Isoform PTPB is expressed in T-cells and in placenta.
  • Sequence similarities

    Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.
    Contains 1 tyrosine-protein phosphatase domain.
  • Cellular localization

    Cytoplasm; Nucleus and Endoplasmic reticulum.
  • Information by UniProt
  • Database links

  • Alternative names

    • Protein tyrosine phosphatase non receptor type 2 antibody
    • PTN2 antibody
    • PTN2_HUMAN antibody
    • PTPN 2 antibody
    • Ptpn2 antibody
    • PTPT antibody
    • T cell protein tyrosine phosphatase antibody
    • T-cell protein-tyrosine phosphatase antibody
    • TC PTP antibody
    • TCELLPTP antibody
    • TCPTP antibody
    • Tyrosine protein phosphatase non receptor type 2 antibody
    • Tyrosine-protein phosphatase non-receptor type 2 antibody
    see all

Images

  • All lanes : Anti-TCPTP antibody - N-terminal (ab226301) at 0.1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 48 kDa


    Exposure time: 10 seconds


    Lysates prepared using NETN lysis buffer.

  • TCPTP was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226301 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226301 at 1 µg/ml.

    Lane 1: ab226301 IP in HeLa whole cell lysate.

    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection:  Chemiluminescence with exposure time of 10 seconds.

References

ab226301 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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