Key features and details
- Rabbit polyclonal to TCPTP - N-terminal
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TCPTP antibody - N-terminal
See all TCPTP primary antibodies
DescriptionRabbit polyclonal to TCPTP - N-terminal
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human TCPTP aa 1-50 (N terminal). The exact sequence is proprietary.
Database link: P17706
- WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226301 was affinity purified using an epitope specific to TCPTP immobilized on solid support.
Our Abpromise guarantee covers the use of ab226301 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2500. Predicted molecular weight: 48 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
Tissue specificityIsoform PTPA is probably the major isoform. Isoform PTPB is expressed in T-cells and in placenta.
Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.
Contains 1 tyrosine-protein phosphatase domain.
Cellular localizationCytoplasm; Nucleus and Endoplasmic reticulum.
- Information by UniProt
- Protein tyrosine phosphatase non receptor type 2 antibody
- PTN2 antibody
- PTN2_HUMAN antibody
All lanes : Anti-TCPTP antibody - N-terminal (ab226301) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 48 kDa
Exposure time: 10 seconds
Lysates prepared using NETN lysis buffer.
TCPTP was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226301 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226301 at 1 µg/ml.
Lane 1: ab226301 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226301 has not yet been referenced specifically in any publications.